8VVN

Cryo-EM structure of a type I ZorAB complex from Shewanella sp. strain ANA-3

8VVN の概要

• エントリーDOI: 10.2210/pdb8vvn/pdb
• EMDBエントリー: 43563
• 分子名称: Chemotaxis protein MotB-related protein, MotA/TolQ/ExbB proton channel domain-containing protein, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: phage defense, zorya, phage, membrane protein complex, membrane protein
• 由来する生物種: Shewanella sp. ANA-3; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 449814.90

構造登録者

Deme, J.C.,Lea, S.M. (登録日: 2024-01-31, 公開日: 2025-02-05, 最終更新日: 2025-06-04)

主引用文献

Mariano, G.,Deme, J.C.,Readshaw, J.J.,Grobbelaar, M.J.,Keenan, M.,El-Masri, Y.,Bamford, L.,Songra, S.,Blower, T.R.,Palmer, T.,Lea, S.M.
Modularity of Zorya defense systems during phage inhibition.
Nat Commun, 16:2344-2344, 2025

PubMed Abstract: Bacteria have evolved an extraordinary diversity of defense systems against bacteriophage (phage) predation. However, the molecular mechanisms underlying these anti-phage systems often remain elusive. Here, we provide mechanistic and structural insights into Zorya phage defense systems. Using cryo-EM structural analyses, we show that the Zorya type I and II core components, ZorA and ZorB, assemble in a 5:2 complex that is similar to inner-membrane ion-driven, rotary motors that power flagellar rotation, type 9 secretion, gliding and the Ton nutrient uptake systems. The ZorAB complex has an elongated cytoplasmic tail assembled by bundling the C-termini of the five ZorA subunits. Mutagenesis demonstrates that peptidoglycan binding by the periplasmic domains of ZorB, the structured cytoplasmic tail of ZorA, and ion flow through the motor is important for function in both type I and II systems. Furthermore, we identify ZorE as the effector module of the Zorya II system, possessing nickase activity. Our work reveals the molecular basis of the activity of Zorya systems and highlights the ZorE nickase as crucial for population-wide immunity in the type II system.

PubMed: 40057510
DOI: 10.1038/s41467-025-57397-2

実験手法

ELECTRON MICROSCOPY (2.2 Å)