8VT5

Crystal structure of human menRNA

Summary for 8VT5

Entry DOI	10.2210/pdb8vt5/pdb
Descriptor	menRNA (2 entities in total)
Functional Keywords	non-coding rnas, cancer, rna, menrna
Biological source	Homo sapiens (human)
Total number of polymer chains	1
Total formula weight	19597.48
Authors	Skeparnias, I.,Zhang, J. (deposition date: 2024-01-25, release date: 2024-07-24, Last modification date: 2024-07-31)
Primary citation	Skeparnias, I.,Zhang, J. Structural basis of NEAT1 lncRNA maturation and menRNA instability. Nat.Struct.Mol.Biol., 2024 Cited by PubMed Abstract: NEAT1 long noncoding RNA orchestrates paraspeckle assembly and impacts tumorigenesis, fertility and immunity. Its maturation requires RNase P cleavage yielding an unstable transfer RNA-like multiple endocrine neoplasia-β tRNA-like transcript (menRNA) due to CCACCA addition. Here we report the crystal structure of human menRNA, which partially mimics tRNAs to drive RNase P and ELAC2 processing. Biophysical analyses uncover an RNA-centric, riboswitch-like mechanism whereby the nascent CCA reshapes the RNA folding landscape and propels a spontaneous conformational isomerization that directs repeat CCA addition, marking the menRNA and defective tRNAs for degradation. PubMed: 39026030 DOI: 10.1038/s41594-024-01361-z PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.69 Å)

Structure validation