8VT5

Crystal structure of human menRNA

8VT5 の概要

• エントリーDOI: 10.2210/pdb8vt5/pdb
• 分子名称: menRNA (2 entities in total)
• 機能のキーワード: non-coding rnas, cancer, rna, menrna
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19597.48

構造登録者

Skeparnias, I.,Zhang, J. (登録日: 2024-01-25, 公開日: 2024-07-24, 最終更新日: 2024-07-31)

主引用文献

Skeparnias, I.,Zhang, J.
Structural basis of NEAT1 lncRNA maturation and menRNA instability.
Nat.Struct.Mol.Biol., 2024

PubMed Abstract: NEAT1 long noncoding RNA orchestrates paraspeckle assembly and impacts tumorigenesis, fertility and immunity. Its maturation requires RNase P cleavage yielding an unstable transfer RNA-like multiple endocrine neoplasia-β tRNA-like transcript (menRNA) due to CCACCA addition. Here we report the crystal structure of human menRNA, which partially mimics tRNAs to drive RNase P and ELAC2 processing. Biophysical analyses uncover an RNA-centric, riboswitch-like mechanism whereby the nascent CCA reshapes the RNA folding landscape and propels a spontaneous conformational isomerization that directs repeat CCA addition, marking the menRNA and defective tRNAs for degradation.

PubMed: 39026030
DOI: 10.1038/s41594-024-01361-z

実験手法

X-RAY DIFFRACTION (2.69 Å)