8VKE

SARS-CoV-2 spike omicron (BA.1) RBD ectodomain dimer-of-trimers in complex with SC27 Fabs

8VKE の概要

• エントリーDOI: 10.2210/pdb8vke/pdb
• EMDBエントリー: 43315
• 分子名称: SC27 Fab heavy chain, SC27 Fab light chain, Spike glycoprotein, ... (4 entities in total)
• 機能のキーワード: sars-cov-2, coronavirus, s, spike, ba.1, omicron, rbd, covid-19, antibody, sc27, fab, virus, viral protein, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 395704.34

構造登録者

Byrne, P.O.,McLellan, J.S. (登録日: 2024-01-09, 公開日: 2024-07-24, 最終更新日: 2025-05-28)

主引用文献

Voss, W.N.,Mallory, M.A.,Byrne, P.O.,Marchioni, J.M.,Knudson, S.A.,Powers, J.M.,Leist, S.R.,Dadonaite, B.,Townsend, D.R.,Kain, J.,Huang, Y.,Satterwhite, E.,Castillo, I.N.,Mattocks, M.,Paresi, C.,Munt, J.E.,Scobey, T.,Seeger, A.,Premkumar, L.,Bloom, J.D.,Georgiou, G.,McLellan, J.S.,Baric, R.S.,Lavinder, J.J.,Ippolito, G.C.
Hybrid immunity to SARS-CoV-2 arises from serological recall of IgG antibodies distinctly imprinted by infection or vaccination.
Cell Rep Med, 5:101668-101668, 2024

PubMed Abstract: We describe the molecular-level composition of polyclonal immunoglobulin G (IgG) anti-spike antibodies from ancestral severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, vaccination, or their combination ("hybrid immunity") at monoclonal resolution. Infection primarily triggers S2/N-terminal domain (NTD)-reactive antibodies, whereas vaccination mainly induces anti-receptor-binding domain (RBD) antibodies. This imprint persists after secondary exposures wherein >60% of ensuing hybrid immunity derives from the original IgG pool. Monoclonal constituents of the original IgG pool can increase breadth, affinity, and prevalence upon secondary exposures, as exemplified by the plasma antibody SC27. Following a breakthrough infection, vaccine-induced SC27 gained neutralization breadth and potency against SARS-CoV-2 variants and zoonotic viruses (half-maximal inhibitory concentration [IC] ∼0.1-1.75 nM) and increased its binding affinity to the protective RBD class 1/4 epitope (dissociation constant [K] < 5 pM). According to polyclonal escape analysis, SC27-like binding patterns are common in SARS-CoV-2 hybrid immunity. Our findings provide a detailed molecular definition of immunological imprinting and show that vaccination can produce class 1/4 (SC27-like) IgG antibodies circulating in the blood.

PubMed: 39094579
DOI: 10.1016/j.xcrm.2024.101668

実験手法

ELECTRON MICROSCOPY (3.1 Å)