8VHT

Cryo EM structure of a soybean CesA3 homotrimer

8VHT の概要

• エントリーDOI: 10.2210/pdb8vht/pdb
• EMDBエントリー: 43241
• 分子名称: Cellulose synthase, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose (2 entities in total)
• 機能のキーワード: cellulose, glycosyltransferase, microfibril, transferase
• 由来する生物種: Glycine max (soybean)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 363192.73

構造登録者

Ho, R.,Palliniti, P.,Zimmer, J. (登録日: 2024-01-02, 公開日: 2025-01-15, 最終更新日: 2025-05-28)

主引用文献

Ho, R.,Pallinti, P.,Wilson, L.F.L.,Wan, Y.,Zimmer, J.
Structure, function and assembly of soybean primary cell wall cellulose synthases.
Elife, 13:-, 2025

PubMed Abstract: Plant cell walls contain a meshwork of cellulose fibers embedded into a matrix of other carbohydrate and non-carbohydrate-based biopolymers. This composite material exhibits extraordinary properties, from stretchable and pliable cell boundaries to solid protective shells. Cellulose, a linear glucose polymer, is synthesized and secreted across the plasma membrane by cellulose synthase (CesA), of which plants express multiple isoforms. Different subsets of CesA isoforms are necessary for primary and secondary cell wall biogenesis. Here, we structurally and functionally characterize the (soybean) primary cell wall CesAs CesA1, CesA3, and CesA6. The CesA isoforms exhibit robust in vitro catalytic activity. Cryo-electron microscopy analyses reveal their assembly into homotrimeric complexes in vitro in which each CesA protomer forms a cellulose-conducting transmembrane channel with a large lateral opening. Biochemical and co-purification analyses demonstrate that different CesA isoforms interact in vitro, leading to synergistic cellulose biosynthesis. Interactions between CesA trimers are only observed between different CesA isoforms and require the class-specific region (CSR). The CSR forms a hook-shaped extension of CesA's catalytic domain at the cytosolic water-lipid interface. Negative stain and cryo-electron microscopy analyses of mixtures of different CesA isoform trimers reveal their side-by-side arrangement into loose clusters. Our data suggest a model by which CesA homotrimers of different isoforms assemble into cellulose synthase complexes to synthesize and secrete multiple cellulose chains for microfibril formation. Inter-trimer interactions are mediated by fuzzy interactions between their CSR extensions.

PubMed: 40365874
DOI: 10.7554/eLife.96704

実験手法

ELECTRON MICROSCOPY (3.2 Å)