8VEE

Cryo-EM structure of antibody T5-1E08 in complex with H7N9 Influenza Hemagglutinin Trimer (A/Shanghai/2/13)

8VEE の概要

• エントリーDOI: 10.2210/pdb8vee/pdb
• 関連するPDBエントリー: 8T1G 8VEB 8VED 8VEF
• EMDBエントリー: 43171
• 分子名称: Hemagglutinin, T5-1E08 Fab heavy chain, T5-1E08 Fab light chain, ... (5 entities in total)
• 機能のキーワード: group 2, influenza, antibody maturation, cryo-em, fab, trimer, spike, hemagglutinin, immune system, viral protein, immune system-viral protein complex, immune system/viral protein
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 337547.61

構造登録者

Cerutti, G.,Casner, R.G.,Shapiro, L. (登録日: 2023-12-18, 公開日: 2025-03-19, 最終更新日: 2025-05-14)

主引用文献

Mantus, G.E.,Cerutti, G.,Chambers, M.,Gillespie, R.A.,Shimberg, G.D.,Spangler, A.,Gorman, J.,Zhou, T.,Shen, C.H.,Kanekiyo, M.,Kwong, P.D.,Shapiro, L.,Andrews, S.F.
Distinct binding modes drive the broad neutralization profile of two persistent influenza hemagglutinin stem-specific antibody lineages.
Structure, 33:869-, 2025

PubMed Abstract: Elicitation of antibodies to the influenza hemagglutinin stem is a critical part of universal influenza vaccine strategies. While numerous broadly reactive stem antibodies have been isolated, our understanding of how these antibodies mature within the human B cell repertoire is limited. Here, we isolated and tracked two stem-specific antibody lineages over a decade in a single participant that received multiple seasonal and pandemic influenza vaccinations. Despite similar binding and neutralization profiles, antibodies from these lineages utilized fundamentally different interactions to engage the central epitope on the influenza stem. Structural analysis of an unmutated common ancestor from one lineage identified critical residues that were the main drivers of increased affinity and breadth to group 1 influenza subtypes. These observations demonstrate the heterogeneous pathways by which stem-specific antibodies can mature within the human B cell repertoire.

PubMed: 40112805
DOI: 10.1016/j.str.2025.02.010

実験手法

ELECTRON MICROSCOPY (3.18 Å)