8VBB

The secreted adhesin EtpA of Enterotoxigenic Escherichia coli in complex with the mouse mAb 1G05

これはPDB形式変換不可エントリーです。

8VBB の概要

• エントリーDOI: 10.2210/pdb8vbb/pdb
• EMDBエントリー: 43119
• 分子名称: EtpA, mouse mAb 1G05 Heavy Chain, mouse mAb 1G05 Light Chain, ... (4 entities in total)
• 機能のキーワード: adhesin, glycoprotein, lectin, antibody-antigen complex, cell adhesion
• 由来する生物種: Escherichia coli ETEC H10407; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 241144.92

構造登録者

Berndsen, Z.T.,Ward, A.B. (登録日: 2023-12-12, 公開日: 2024-09-04, 最終更新日: 2025-03-19)

主引用文献

Berndsen, Z.T.,Akhtar, M.,Thapa, M.,Vickers, T.J.,Schmitz, A.,Torres, J.L.,Baboo, S.,Kumar, P.,Khatoon, N.,Sheikh, A.,Hamrick, M.,Diedrich, J.K.,Martinez-Bartolome, S.,Garrett, P.T.,Yates 3rd, J.R.,Turner, J.S.,Laird, R.M.,Poly, F.,Porter, C.K.,Copps, J.,Ellebedy, A.H.,Ward, A.B.,Fleckenstein, J.M.
Repeat modules and N-linked glycans define structure and antigenicity of a critical enterotoxigenic E. coli adhesin.
Plos Pathog., 20:e1012241-e1012241, 2024

PubMed Abstract: Enterotoxigenic Escherichia coli (ETEC) cause hundreds of millions of cases of infectious diarrhea annually, predominantly in children from low-middle income regions. Notably, in children, as well as volunteers challenged with ETEC, diarrheal severity is significantly increased in blood group A (bgA) individuals. EtpA, is a secreted glycoprotein adhesin that functions as a blood group A lectin to promote critical interactions between ETEC and blood group A glycans on intestinal epithelia for effective bacterial adhesion and toxin delivery. EtpA is highly immunogenic resulting in robust antibody responses following natural infection and experimental challenge of volunteers with ETEC. To understand how EtpA directs ETEC-blood group A interactions and stimulates adaptive immunity, we mutated EtpA, mapped its glycosylation by mass-spectrometry (MS), isolated polyclonal (pAbs) and monoclonal antibodies (mAbs) from vaccinated mice and ETEC-infected volunteers, and determined structures of antibody-EtpA complexes by cryo-electron microscopy. Both bgA and mAbs that inhibited EtpA-bgA interactions and ETEC adhesion, bound to the C-terminal repeat domain highlighting this region as crucial for ETEC pathogen-host interaction. MS analysis uncovered extensive and heterogeneous N-linked glycosylation of EtpA and cryo-EM structures revealed that mAbs directly engage these unique glycan containing epitopes. Finally, electron microscopy-based polyclonal epitope mapping revealed antibodies targeting numerous distinct epitopes on N and C-terminal domains, suggesting that EtpA vaccination generates responses against neutralizing and decoy regions of the molecule. Collectively, we anticipate that these data will inform our general understanding of pathogen-host glycan interactions and adaptive immunity relevant to rational vaccine subunit design.

PubMed: 39283948
DOI: 10.1371/journal.ppat.1012241

実験手法

ELECTRON MICROSCOPY (3.97 Å)