8VB7
Kinetic intermediate states of HIV-1 RT DNA synthesis captured by cryo-EM
8VB7 の概要
エントリーDOI | 10.2210/pdb8vb7/pdb |
EMDBエントリー | 43115 |
分子名称 | HIV-1 reverse transcriptase/ribonuclease H P66 subunit, HIV-1 reverse transcriptase P51 subunit, DNA (38-MER), ... (5 entities in total) |
機能のキーワード | reverse transcription, time-resolved, hiv-1, cryo-em, transcription, transferase-dna complex, transferase/dna |
由来する生物種 | Human immunodeficiency virus 1 詳細 |
タンパク質・核酸の鎖数 | 3 |
化学式量合計 | 128304.15 |
構造登録者 | Vergara, S.,Zhou, X.,Santiago, U.,Conway, J.F.,Sluis-Cremer, N.,Calero, G. (登録日: 2023-12-12, 公開日: 2024-12-11, 最終更新日: 2024-12-18) |
主引用文献 | Vergara, S.,Zhou, X.,Santiago, U.,Alaoui-El-Azher, M.,Conway, J.F.,Sluis-Cremer, N.,Calero, G. Structural basis of deoxynucleotide addition by HIV-1 RT during reverse transcription. Nat Commun, 15:10553-10553, 2024 Cited by PubMed Abstract: Reverse transcription of the retroviral RNA genome into DNA is an integral step during HIV-1 replication. Despite a wealth of structural information on reverse transcriptase (RT), we lack insight into the intermediate states of DNA synthesis. Using catalytically active substrates, and a blot/diffusion cryo-electron microscopy approach, we capture 11 structures encompassing reactant, intermediate and product states of dATP addition by RT at 2.2 to 3.0 Å resolution. In the reactant state, dATP binding to RT-template/primer involves a single Mg (site B) inducing formation of a negatively charged pocket where a second floating Mg can bind (site A). During the intermediate state, the α-phosphate oxygen from a previously unobserved dATP conformer aligns with site A Mg and the primer 3'-OH for nucleophilic attack. The product state, comprises two substrate conformations including an incorporated dAMP with the pyrophosphate leaving group coordinated by metal B and stabilized through H-bonds. Moreover, K220 mutants significantly impact the rate of dNTP incorporation by RT and HIV-1 replication capacity. This work sheds light into the dynamic components of a reaction that is central to HIV-1 replication. PubMed: 39632888DOI: 10.1038/s41467-024-54618-y 主引用文献が同じPDBエントリー |
実験手法 | ELECTRON MICROSCOPY (2.5 Å) |
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