8VAE

Cryogenic electron microscopy structure of human serum albumin in complex with salicylic acid

8VAE の概要

• エントリーDOI: 10.2210/pdb8vae/pdb
• EMDBエントリー: 43089
• 分子名称: Serum albumin, 2-HYDROXYBENZOIC ACID (2 entities in total)
• 機能のキーワード: human serum albumin, salicylic acid, transport protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 66985.58

構造登録者

Catalano, C.,Lucier, K.W.,To, D.,Senko, S.,Tran, N.L.,Farwell, A.C.,Silva, S.M.,Dip, P.V.,Poweleit, N.,Scapin, G. (登録日: 2023-12-11, 公開日: 2024-06-26, 最終更新日: 2024-10-16)

主引用文献

Catalano, C.,Lucier, K.W.,To, D.,Senko, S.,Tran, N.L.,Farwell, A.C.,Silva, S.M.,Dip, P.V.,Poweleit, N.,Scapin, G.
The CryoEM structure of human serum albumin in complex with ligands.
J.Struct.Biol., 216:108105-108105, 2024

PubMed Abstract: Human serum albumin (HSA) is the most prevalent plasma protein in the human body, accounting for 60 % of the total plasma protein. HSA plays a major pharmacokinetic function, serving as a facilitator in the distribution of endobiotics and xenobiotics within the organism. In this paper we report the cryoEM structures of HSA in the apo form and in complex with two ligands (salicylic acid and teniposide) at a resolution of 3.5, 3.7 and 3.4 Å, respectively. We expand upon previously published work and further demonstrate that sub-4 Å maps of ∼60 kDa proteins can be routinely obtained using a 200 kV microscope, employing standard workflows. Most importantly, these maps allowed for the identification of small molecule ligands, emphasizing the practical applicability of this methodology and providing a starting point for subsequent computational modeling and in silico optimization.

PubMed: 38852682
DOI: 10.1016/j.jsb.2024.108105

実験手法

ELECTRON MICROSCOPY (3.7 Å)