8V41

CryoEM Structure of Diffocin - postcontracted - Baseplate - transitional state

これはPDB形式変換不可エントリーです。

8V41 の概要

• エントリーDOI: 10.2210/pdb8v41/pdb
• EMDBエントリー: 42963
• 分子名称: TRI-2 (CD1371), Sheath (CD1363), Sheath initiator (CD1370), ... (4 entities in total)
• 機能のキーワード: phage tail-like, bacteriocin, baseplate, post-contraction, virus like particle
• 由来する生物種: Clostridioides difficile; 詳細
• タンパク質・核酸の鎖数: 42
• 化学式量合計: 1440211.79

構造登録者

Cai, X.Y.,He, Y.,Zhou, Z.H. (登録日: 2023-11-28, 公開日: 2024-08-28)

主引用文献

Cai, X.,He, Y.,Yu, I.,Imani, A.,Scholl, D.,Miller, J.F.,Zhou, Z.H.
Atomic structures of a bacteriocin targeting Gram-positive bacteria.
Nat Commun, 15:7057-7057, 2024

PubMed Abstract: Due to envelope differences between Gram-positive and Gram-negative bacteria, engineering precision bactericidal contractile nanomachines requires atomic-level understanding of their structures; however, only those killing Gram-negative bacteria are currently known. Here, we report the atomic structures of an engineered diffocin, a contractile syringe-like molecular machine that kills the Gram-positive bacterium Clostridioides difficile. Captured in one pre-contraction and two post-contraction states, each structure fashions six proteins in the bacteria-targeting baseplate, two proteins in the energy-storing trunk, and a collar linking the sheath with the membrane-penetrating tube. Compared to contractile machines targeting Gram-negative bacteria, major differences reside in the baseplate and contraction magnitude, consistent with target envelope differences. The multifunctional hub-hydrolase protein connects the tube and baseplate and is positioned to degrade peptidoglycan during penetration. The full-length tape measure protein forms a coiled-coil helix bundle homotrimer spanning the entire diffocin. Our study offers mechanical insights and principles for designing potent protein-based precision antibiotics.

PubMed: 39152109
DOI: 10.1038/s41467-024-51038-w

実験手法

ELECTRON MICROSCOPY (5.6 Å)