8URU

Spo11 core complex with hairpin DNA

8URU の概要

• エントリーDOI: 10.2210/pdb8uru/pdb
• EMDBエントリー: 42501
• 分子名称: Meiosis-specific protein SPO11, Meiotic recombination protein REC102, Antiviral protein SKI8, ... (6 entities in total)
• 機能のキーワード: spo11, rec102, rec104, ski8, dna binding, cross over., dna binding protein
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 160244.44

構造登録者

Yu, Y.,Patel, D.J. (登録日: 2023-10-26, 公開日: 2024-06-26, 最終更新日: 2025-05-21)

主引用文献

Yu, Y.,Wang, J.,Liu, K.,Zheng, Z.,Arter, M.,Claeys Bouuaert, C.,Pu, S.,Patel, D.J.,Keeney, S.
Cryo-EM structures of the Spo11 core complex bound to DNA.
Nat.Struct.Mol.Biol., 32:113-124, 2025

PubMed Abstract: DNA double-strand breaks that initiate meiotic recombination are formed by the topoisomerase-relative enzyme Spo11, supported by conserved auxiliary factors. Because high-resolution structural data have not been available, many questions remain about the architecture of Spo11 and its partners and how they engage with DNA. We report cryo-electron microscopy structures at up to 3.3-Å resolution of DNA-bound core complexes of Saccharomyces cerevisiae Spo11 with Rec102, Rec104 and Ski8. In these structures, monomeric core complexes make extensive contacts with the DNA backbone and with the recessed 3'-OH and first 5' overhanging nucleotide, establishing the molecular determinants of DNA end-binding specificity and providing insight into DNA cleavage preferences in vivo. The structures of individual subunits and their interfaces, supported by functional data in yeast, provide insight into the role of metal ions in DNA binding and uncover unexpected structural variation in homologs of the Top6BL component of the core complex.

PubMed: 39304764
DOI: 10.1038/s41594-024-01382-8

実験手法

ELECTRON MICROSCOPY (3.7 Å)