8URB

Porcine epidemic diarrhea virus complete core polymerase complex

8URB の概要

• エントリーDOI: 10.2210/pdb8urb/pdb
• 関連するPDBエントリー: 8G6R
• EMDBエントリー: 42488
• 分子名称: nsp12, nsp8, nsp7, ... (6 entities in total)
• 機能のキーワード: coronavirus, nsp12, rna polymerase, pedv, replication, transferase-rna complex, transferase/rna
• 由来する生物種: Porcine epidemic diarrhea virus; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 189998.60

構造登録者

Anderson, T.K.,Kirchdoerfer, R.N. (登録日: 2023-10-25, 公開日: 2023-11-15)

主引用文献

Anderson, T.K.,Hoferle, P.J.,Lee, K.W.,Coon, J.J.,Kirchdoerfer, R.N.
An alphacoronavirus polymerase structure reveals conserved co-factor functions.
Biorxiv, 2023

PubMed Abstract: Coronaviruses are a diverse subfamily of viruses containing pathogens of humans and animals. This subfamily of viruses replicates their RNA genomes using a core polymerase complex composed of viral non-structural proteins: nsp7, nsp8 and nsp12. Most of our understanding of coronavirus molecular biology comes from the betacoronaviruses like SARS-CoV and SARS-CoV-2, the latter of which is the causative agent of COVID-19. In contrast, members of the alphacoronavirus genus are relatively understudied despite their importance in human and animal health. Here we have used cryo-electron microscopy to determine the structure of the alphacoronavirus porcine epidemic diarrhea virus (PEDV) core polymerase complex bound to RNA. Our structure shows an unexpected nsp8 stoichiometry in comparison to other published coronavirus polymerase structures. Biochemical analysis shows that the N-terminal extension of one nsp8 is not required for RNA synthesis for alpha and betacoronaviruses as previously hypothesized. Our work shows the importance of studying diverse coronaviruses to reveal aspects of coronavirus replication while also identifying areas of conservation to be targeted by antiviral drugs.

PubMed: 36993498
DOI: 10.1101/2023.03.15.532841

実験手法

ELECTRON MICROSCOPY (3.4 Å)