8UC3

Cryo-EM structure of the AlbAB cyclodipeptide oxidase enzyme filament

8UC3 の概要

• エントリーDOI: 10.2210/pdb8uc3/pdb
• EMDBエントリー: 42114
• 分子名称: Albonoursin synthase, Protein AlbB, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: cyclodipeptide oxidase, cyclic dipeptide oxidase, nitroreductase-like, enzyme filament, flavoenzyme, oxidoreductase
• 由来する生物種: Streptomyces noursei ATCC 11455; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 66221.59

構造登録者

Andreas, M.P.,Giessen, T.W. (登録日: 2023-09-25, 公開日: 2024-05-15, 最終更新日: 2024-10-16)

主引用文献

Andreas, M.P.,Giessen, T.W.
Cyclodipeptide oxidase is an enzyme filament.
Nat Commun, 15:3574-3574, 2024

PubMed Abstract: Modified cyclic dipeptides represent a widespread class of secondary metabolites with diverse pharmacological activities, including antibacterial, antifungal, and antitumor. Here, we report the structural characterization of the Streptomyces noursei enzyme AlbAB, a cyclodipeptide oxidase (CDO) carrying out α,β-dehydrogenations during the biosynthesis of the antibiotic albonoursin. We show that AlbAB is a megadalton heterooligomeric enzyme filament containing covalently bound flavin mononucleotide cofactors. We highlight that AlbAB filaments consist of alternating dimers of AlbA and AlbB and that enzyme activity is crucially dependent on filament formation. We show that AlbA-AlbB interactions are highly conserved suggesting that other CDO-like enzymes are likely enzyme filaments. As CDOs have been employed in the structural diversification of cyclic dipeptides, our results will be useful for future applications of CDOs in biocatalysis and chemoenzymatic synthesis.

PubMed: 38678027
DOI: 10.1038/s41467-024-48030-9

実験手法

ELECTRON MICROSCOPY (2.78 Å)