8UBB

Diversity-generating retroelement (DGR) ribonucleoprotein reverse transcriptase - Active State (N-empty) 1b

8UBB の概要

• エントリーDOI: 10.2210/pdb8ubb/pdb
• EMDBエントリー: 42081
• 分子名称: Reverse transcriptase, Avd, Diversity-generating retroelement (DGR) RNA avd, ... (5 entities in total)
• 機能のキーワード: reverse transcriptase, ribonucleoprotein, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Bordetella phage BPP-1; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 259553.21

構造登録者

Biswas, T.,Handa, S.,Ghosh, P. (登録日: 2023-09-22, 公開日: 2024-10-23, 最終更新日: 2025-03-12)

主引用文献

Handa, S.,Biswas, T.,Chakraborty, J.,Ghosh, G.,Paul, B.G.,Ghosh, P.
RNA control of reverse transcription in a diversity-generating retroelement.
Nature, 638:1122-1129, 2025

PubMed Abstract: Diversity-generating retroelements (DGRs) create massive protein sequence variation (up to 10) in ecologically diverse microorganisms. A recent survey identified around 31,000 DGRs from more than 1,500 bacterial and archaeal genera, constituting more than 90 environment types. DGRs are especially enriched in the human gut microbiome and nano-sized microorganisms that seem to comprise most microbial life and maintain DGRs despite reduced genomes. DGRs are also implicated in the emergence of multicellularity. Variation occurs during reverse transcription of a protein-encoding RNA template coupled to misincorporation at adenosines. In the prototypical Bordetella bacteriophage DGR, the template must be surrounded by upstream and downstream RNA segments for complementary DNA synthesis to be carried out by a complex of the DGR reverse transcriptase bRT and associated protein Avd. The function of the surrounding RNA was unknown. Here we show through cryogenic electron microscopy that this RNA envelops bRT and lies over the barrel-shaped Avd, forming an intimate ribonucleoprotein. An abundance of essential interactions in the ribonucleoprotein precisely position an RNA homoduplex in the bRT active site for initiation of reverse transcription. Our results explain how the surrounding RNA primes complementary DNA synthesis, promotes processivity, terminates polymerization and strictly limits mutagenesis to specific proteins through mechanisms that are probably conserved in DGRs belonging to distant taxa.

PubMed: 39779855
DOI: 10.1038/s41586-024-08405-w

実験手法

ELECTRON MICROSCOPY (3.23 Å)