8U9O

Solution structure of RsgI9 CRE domain from C. thermocellum

8U9O の概要

• エントリーDOI: 10.2210/pdb8u9o/pdb
• NMR情報: BMRB: 52129
• 分子名称: Anti-sigma-I factor RsgI9 (2 entities in total)
• 機能のキーワード: rsgi, cellulosome, anti-sigma factor, auto-proteolysis, signaling protein
• 由来する生物種: Acetivibrio thermocellus DSM 1313; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20037.56

構造登録者

Takayesu, A.,Mahoney, B.J.,Clubb, R.T. (登録日: 2023-09-19, 公開日: 2024-04-24, 最終更新日: 2024-07-10)

主引用文献

Takayesu, A.,Mahoney, B.J.,Goring, A.K.,Jessup, T.,Ogorzalek Loo, R.R.,Loo, J.A.,Clubb, R.T.
Insight into the autoproteolysis mechanism of the RsgI9 anti-sigma factor from Clostridium thermocellum.
Proteins, 92:946-958, 2024

PubMed Abstract: Clostridium thermocellum is a potential microbial platform to convert abundant plant biomass to biofuels and other renewable chemicals. It efficiently degrades lignocellulosic biomass using a surface displayed cellulosome, a megadalton sized multienzyme containing complex. The enzymatic composition and architecture of the cellulosome is controlled by several transmembrane biomass-sensing RsgI-type anti-σ factors. Recent studies suggest that these factors transduce signals from the cell surface via a conserved RsgI extracellular (CRE) domain (also called a periplasmic domain) that undergoes autoproteolysis through an incompletely understood mechanism. Here we report the structure of the autoproteolyzed CRE domain from the C. thermocellum RsgI9 anti-σ factor, revealing that the cleaved fragments forming this domain associate to form a stable α/β/α sandwich fold. Based on AlphaFold2 modeling, molecular dynamics simulations, and tandem mass spectrometry, we propose that a conserved Asn-Pro bond in RsgI9 autoproteolyzes via a succinimide intermediate whose formation is promoted by a conserved hydrogen bond network holding the scissile peptide bond in a strained conformation. As other RsgI anti-σ factors share sequence homology to RsgI9, they likely autoproteolyze through a similar mechanism.

PubMed: 38597224
DOI: 10.1002/prot.26690

実験手法

SOLUTION NMR