8U95

The structure of myosin heavy chain from Drosophila melanogaster flight muscle thick filaments

8U95 の概要

• エントリーDOI: 10.2210/pdb8u95/pdb
• 関連するPDBエントリー: 8U8H
• EMDBエントリー: 42024
• 分子名称: Myosin heavy chain, isoform U (1 entity in total)
• 機能のキーワード: striated muscle, regulatory protein, myosin, flightin, myofilin, stretchin, structural protein
• 由来する生物種: Drosophila melanogaster (fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 446521.59

構造登録者

Abbasi Yeganeh, F.,Rastegarpouyani, H.,Li, J.,Taylor, K.A. (登録日: 2023-09-18, 公開日: 2023-10-18, 最終更新日: 2025-05-14)

主引用文献

Abbasi Yeganeh, F.,Rastegarpouyani, H.,Li, J.,Taylor, K.A.
Structure of the Drosophila melanogaster Flight Muscle Myosin Filament at 4.7 angstrom Resolution Reveals New Details of Non-Myosin Proteins.
Int J Mol Sci, 24:-, 2023

PubMed Abstract: Striated muscle thick filaments are composed of myosin II and several non-myosin proteins which define the filament length and modify its function. Myosin II has a globular N-terminal motor domain comprising its catalytic and actin-binding activities and a long α-helical, coiled tail that forms the dense filament backbone. Myosin alone polymerizes into filaments of irregular length, but striated muscle thick filaments have defined lengths that, with thin filaments, define the sarcomere structure. The motor domain structure and function are well understood, but the myosin filament backbone is not. Here we report on the structure of the flight muscle thick filaments from at 4.7 Å resolution, which eliminates previous ambiguities in non-myosin densities. The full proximal S2 region is resolved, as are the connecting densities between the Ig domains of stretchin-klp. The proteins, flightin, and myofilin are resolved in sufficient detail to build an atomic model based on an AlphaFold prediction. Our results suggest a method by which flightin and myofilin cooperate to define the structure of the thick filament and explains a key myosin mutation that affects flightin incorporation. is a genetic model organism for which our results can define strategies for functional testing.

PubMed: 37834384
DOI: 10.3390/ijms241914936

実験手法

ELECTRON MICROSCOPY (4.7 Å)