8U8H
The structure of flightin within myosin thick filaments from Drosophila melanogaster flight muscle
Summary for 8U8H
| Entry DOI | 10.2210/pdb8u8h/pdb |
| Related | 8U95 |
| EMDB information | 42024 |
| Descriptor | Flightin (1 entity in total) |
| Functional Keywords | striated muscle, regulatory protein, myosin, flightin, myofilin, stretchin, structural protein |
| Biological source | Drosophila melanogaster (fruit fly) |
| Total number of polymer chains | 1 |
| Total formula weight | 20682.81 |
| Authors | Abbasi Yeganeh, F.,Rastegarpouyani, H.,Li, J.,Taylor, K.A. (deposition date: 2023-09-18, release date: 2023-10-18, Last modification date: 2023-11-01) |
| Primary citation | Abbasi Yeganeh, F.,Rastegarpouyani, H.,Li, J.,Taylor, K.A. Structure of the Drosophila melanogaster Flight Muscle Myosin Filament at 4.7 angstrom Resolution Reveals New Details of Non-Myosin Proteins. Int J Mol Sci, 24:-, 2023 Cited by PubMed Abstract: Striated muscle thick filaments are composed of myosin II and several non-myosin proteins which define the filament length and modify its function. Myosin II has a globular N-terminal motor domain comprising its catalytic and actin-binding activities and a long α-helical, coiled tail that forms the dense filament backbone. Myosin alone polymerizes into filaments of irregular length, but striated muscle thick filaments have defined lengths that, with thin filaments, define the sarcomere structure. The motor domain structure and function are well understood, but the myosin filament backbone is not. Here we report on the structure of the flight muscle thick filaments from at 4.7 Å resolution, which eliminates previous ambiguities in non-myosin densities. The full proximal S2 region is resolved, as are the connecting densities between the Ig domains of stretchin-klp. The proteins, flightin, and myofilin are resolved in sufficient detail to build an atomic model based on an AlphaFold prediction. Our results suggest a method by which flightin and myofilin cooperate to define the structure of the thick filament and explains a key myosin mutation that affects flightin incorporation. is a genetic model organism for which our results can define strategies for functional testing. PubMed: 37834384DOI: 10.3390/ijms241914936 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.7 Å) |
Structure validation
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