8U42

OvsA from Halomonas utahensis, a selenoxide synthase involved in ovoselenol biosynthesis

8U42 の概要

• エントリーDOI: 10.2210/pdb8u42/pdb
• 関連するPDBエントリー: 8U41
• 分子名称: Selenoxide synthase OvsA, FE (III) ION, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: oxidoreductase, ovoselenol, selenium, selenoxide, nonheme iron
• 由来する生物種: Halomonas utahensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 110003.73

構造登録者

Ireland, K.A.,Davis, K.M. (登録日: 2023-09-08, 公開日: 2024-07-17, 最終更新日: 2024-11-13)

主引用文献

Kayrouz, C.M.,Ireland, K.A.,Ying, V.Y.,Davis, K.M.,Seyedsayamdost, M.R.
Discovery of the selenium-containing antioxidant ovoselenol derived from convergent evolution.
Nat.Chem., 16:1868-1875, 2024

PubMed Abstract: Selenium is an essential micronutrient, but its presence in biology has been limited to protein and nucleic acid biopolymers. The recent identification of a biosynthetic pathway for selenium-containing small molecules suggests that there is a larger family of selenometabolites that remains to be discovered. Here we identify a recently evolved branch of abundant and uncharacterized metalloenzymes that we predict are involved in selenometabolite biosynthesis using a bioinformatic search strategy that relies on the mapping of composite active site motifs. Biochemical studies confirm this prediction and show that these enzymes form an unusual C-Se bond onto histidine, thus giving rise to a distinct selenometabolite and potent antioxidant that we have termed ovoselenol. Aside from providing insights into the evolution of this enzyme class and the structural basis of C-Se bond formation, our work offers a blueprint for charting the microbial selenometabolome in the future.

PubMed: 39143299
DOI: 10.1038/s41557-024-01600-2

実験手法

X-RAY DIFFRACTION (2 Å)