8U22

A Highly Stable Variant of Corynactis Californica Green Fluorescent Protein, ccGFP 7

8U22 の概要

• エントリーDOI: 10.2210/pdb8u22/pdb
• 分子名称: Green Fluorescent Protein Variant #7, ccGFP 7 (2 entities in total)
• 機能のキーワード: florescent protein, fluorescent protein
• 由来する生物種: Corynactis californica
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25037.45

構造登録者

Hung, L.-W.,Terwilliger, T.C.,Waldo, G.,Nguyen, H.B. (登録日: 2023-09-05, 公開日: 2024-01-31, 最終更新日: 2024-10-23)

主引用文献

Hung, L.W.,Terwilliger, T.C.,Waldo, G.S.,Nguyen, H.B.
Engineering highly stable variants of Corynactis californica green fluorescent proteins.
Protein Sci., 33:e4886-e4886, 2024

PubMed Abstract: Fluorescent proteins (FPs) are versatile biomarkers that facilitate effective detection and tracking of macromolecules of interest in real time. Engineered FPs such as superfolder green fluorescent protein (sfGFP) and superfolder Cherry (sfCherry) have exceptional refolding capability capable of delivering fluorescent readout in harsh environments where most proteins lose their native functions. Our recent work on the development of a split FP from a species of strawberry anemone, Corynactis californica, delivered pairs of fragments with up to threefold faster complementation than split GFP. We present the biophysical, biochemical, and structural characteristics of five full-length variants derived from these split C. californica GFP (ccGFP). These ccGFP variants are more tolerant under chemical denaturation with up to 8 kcal/mol lower unfolding free energy than that of the sfGFP. It is likely that some of these ccGFP variants could be suitable as biomarkers under more adverse environments where sfGFP fails to survive. A structural analysis suggests explanations of the variations in stabilities among the ccGFP variants.

PubMed: 38151801
DOI: 10.1002/pro.4886

実験手法

X-RAY DIFFRACTION (1.8 Å)