8U1B

C-terminal LRRK2 bound to E11 DARPin

Summary for 8U1B

• Entry DOI: 10.2210/pdb8u1b/pdb
• EMDB information: 41806
• Descriptor: Leucine-rich repeat serine/threonine-protein kinase 2, E11 DARPin (2 entities in total)
• Functional Keywords: kinase, complex, darpins, protein binding
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 155827.57

Authors

Sanz-Murillo, M.,Mathea, S.,Dederer, V.,Knapp, S.,Leschziner, A. (deposition date: 2023-08-31, release date: 2024-09-04, Last modification date: 2025-03-26)

Primary citation

Dederer, V.,Sanz Murillo, M.,Karasmanis, E.P.,Hatch, K.S.,Chatterjee, D.,Preuss, F.,Abdul Azeez, K.R.,Nguyen, L.V.,Galicia, C.,Dreier, B.,Pluckthun, A.,Versees, W.,Mathea, S.,Leschziner, A.E.,Reck-Peterson, S.L.,Knapp, S.
A designed ankyrin-repeat protein that targets Parkinson's disease-associated LRRK2.
J.Biol.Chem., 300:107469-107469, 2024

PubMed Abstract: Leucine rich repeat kinase 2 (LRRK2) is a large multidomain protein containing two catalytic domains, a kinase and a GTPase, as well as protein interactions domains, including a WD40 domain. The association of increased LRRK2 kinase activity with both the familial and sporadic forms of Parkinson's disease has led to an intense interest in determining its cellular function. However, small molecule probes that can bind to LRRK2 and report on or affect its cellular activity are needed. Here, we report the identification and characterization of the first high-affinity LRRK2-binding designed ankyrin-repeat protein (DARPin), named E11. Using cryo-EM, we show that DARPin E11 binds to the LRRK2 WD40 domain. LRRK2 bound to DARPin E11 showed improved behavior on cryo-EM grids, resulting in higher resolution LRRK2 structures. DARPin E11 did not affect the catalytic activity of a truncated form of LRRK2 in vitro but decreased the phosphorylation of Rab8A, a LRRK2 substrate, in cells. We also found that DARPin E11 disrupts the formation of microtubule-associated LRRK2 filaments in cells, which are known to require WD40-based dimerization. Thus, DARPin E11 is a new tool to explore the function and dysfunction of LRRK2 and guide the development of LRRK2 kinase inhibitors that target the WD40 domain instead of the kinase.

PubMed: 38876305
DOI: 10.1016/j.jbc.2024.107469

Experimental method

ELECTRON MICROSCOPY (3.7 Å)