8TXP

Crystal structure of 05.GC.w13.01 Fab in complex with H1 HA from A/California/04/2009(H1N1)

8TXP の概要

• エントリーDOI: 10.2210/pdb8txp/pdb
• 分子名称: Hemagglutinin, GC_w13_A, Fab heavy chain, GC_w13_A, Fab light chain, ... (6 entities in total)
• 機能のキーワード: h1n1, antibody, hemagglutinin, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 106503.62

構造登録者

Lin, T.H.,Moore, N.,Wilson, I.A. (登録日: 2023-08-24, 公開日: 2024-06-26, 最終更新日: 2024-11-06)

主引用文献

McIntire, K.M.,Meng, H.,Lin, T.H.,Kim, W.,Moore, N.E.,Han, J.,McMahon, M.,Wang, M.,Malladi, S.K.,Mohammed, B.M.,Zhou, J.Q.,Schmitz, A.J.,Hoehn, K.B.,Carreno, J.M.,Yellin, T.,Suessen, T.,Middleton, W.D.,Teefey, S.A.,Presti, R.M.,Krammer, F.,Turner, J.S.,Ward, A.B.,Wilson, I.A.,Kleinstein, S.H.,Ellebedy, A.H.
Maturation of germinal center B cells after influenza virus vaccination in humans.
J.Exp.Med., 221:-, 2024

PubMed Abstract: Germinal centers (GC) are microanatomical lymphoid structures where affinity-matured memory B cells and long-lived bone marrow plasma cells are primarily generated. It is unclear how the maturation of B cells within the GC impacts the breadth and durability of B cell responses to influenza vaccination in humans. We used fine needle aspiration of draining lymph nodes to longitudinally track antigen-specific GC B cell responses to seasonal influenza vaccination. Antigen-specific GC B cells persisted for at least 13 wk after vaccination in two out of seven individuals. Monoclonal antibodies (mAbs) derived from persisting GC B cell clones exhibit enhanced binding affinity and breadth to influenza hemagglutinin (HA) antigens compared with related GC clonotypes isolated earlier in the response. Structural studies of early and late GC-derived mAbs from one clonal lineage in complex with H1 and H5 HAs revealed an altered binding footprint. Our study shows that inducing sustained GC reactions after influenza vaccination in humans supports the maturation of responding B cells.

PubMed: 38935072
DOI: 10.1084/jem.20240668

実験手法

X-RAY DIFFRACTION (2.75 Å)