8TXC

Characterization of the Chlamydomonas Flagellar Mastigoneme Filament Subunit MST1 Structure at 3.9 angstrom

8TXC の概要

• エントリーDOI: 10.2210/pdb8txc/pdb
• 関連するPDBエントリー: 8XTC
• EMDBエントリー: 41679 41680
• 分子名称: Mastigoneme-like protein (1 entity in total)
• 機能のキーワード: fibrous flagellar hairs, structural protein
• 由来する生物種: Chlamydomonas reinhardtii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 205404.20

構造登録者

Yue, W.,Kai, Z. (登録日: 2023-08-23, 公開日: 2023-10-25, 最終更新日: 2024-10-09)

主引用文献

Wang, Y.,Yang, J.,Hu, F.,Yang, Y.,Huang, K.,Zhang, K.
Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes.
J.Cell Biol., 222:-, 2023

PubMed Abstract: Mastigonemes are thread-like structures adorning the flagella of protists. In Chlamydomonas reinhardtii, filamentous mastigonemes find their roots in the flagella's distal region, associated with the channel protein PKD2, implying their potential contribution to external signal sensing and flagellar motility control. Here, we present the single-particle cryo-electron microscopy structure of the mastigoneme at 3.4 Å. The filament unit, MST1, consists of nine immunoglobulin-like domains and six Sushi domains, trailed by an elastic polyproline-II helix. Our structure demonstrates that MST1 subunits are periodically assembled to form a centrosymmetric, non-polar filament. Intriguingly, numerous clustered disulfide bonds within a ladder-like spiral configuration underscore structural resilience. While defects in the mastigoneme structure did not noticeably affect general attributes of cell swimming, they did impact specific swimming properties, particularly under varied environmental conditions such as redox shifts and heightened viscosity. Our findings illuminate the potential role of mastigonemes in flagellar motility and suggest their involvement in diverse environmental responses.

PubMed: 37882754
DOI: 10.1083/jcb.202301066

実験手法

ELECTRON MICROSCOPY (3.9 Å)