8TUX

Capsid of mature PP7 virion with 3'end region of PP7 genomic RNA

これはPDB形式変換不可エントリーです。

8TUX の概要

• エントリーDOI: 10.2210/pdb8tux/pdb
• EMDBエントリー: 41632
• 分子名称: 3'end of PP7 genomic RNA, Maturation protein A, Capsid protein (3 entities in total)
• 機能のキーワード: maturation protein, pp7, pepevirus, viral protein
• 由来する生物種: Pseudomonas phage PP7; 詳細
• タンパク質・核酸の鎖数: 181
• 化学式量合計: 2607395.94

構造登録者

Thongchol, J.,Zhang, J.,Zeng, L. (登録日: 2023-08-17, 公開日: 2024-03-13, 最終更新日: 2024-10-30)

主引用文献

Thongchol, J.,Yu, Z.,Harb, L.,Lin, Y.,Koch, M.,Theodore, M.,Narsaria, U.,Shaevitz, J.,Gitai, Z.,Wu, Y.,Zhang, J.,Zeng, L.
Removal of Pseudomonas type IV pili by a small RNA virus.
Science, 384:eadl0635-eadl0635, 2024

PubMed Abstract: The retractile type IV pilus (T4P) is important for virulence of the opportunistic human pathogen . The single-stranded RNA (ssRNA) phage PP7 binds to T4P and is brought to the cell surface through pilus retraction. Using fluorescence microscopy, we discovered that PP7 detaches T4P, which impairs cell motility and restricts the pathogen's virulence. Using cryo-electron microscopy, mutagenesis, optical trapping, and Langevin dynamics simulation, we resolved the structure of PP7, T4P, and the PP7/T4P complex and showed that T4P detachment is driven by the affinity between the phage maturation protein and its bound pilin, plus the pilus retraction force and speed, and pilus bending. Pilus detachment may be widespread among other ssRNA phages and their retractile pilus systems and offers new prospects for antibacterial prophylaxis and therapeutics.

PubMed: 38574145
DOI: 10.1126/science.adl0635

実験手法

ELECTRON MICROSCOPY (3.9 Å)