8TUM

Type IV pilus from Pseudomonas PAO1 strain

8TUM の概要

• エントリーDOI: 10.2210/pdb8tum/pdb
• EMDBエントリー: 41625 41780
• 分子名称: Type IV major pilin protein PilA (1 entity in total)
• 機能のキーワード: type iv pilus, t4p, pao1, membrane protein
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 238062.22

構造登録者

Thongchol, J.,Zhang, J.,Zeng, L. (登録日: 2023-08-16, 公開日: 2024-03-13, 最終更新日: 2024-10-23)

主引用文献

Thongchol, J.,Yu, Z.,Harb, L.,Lin, Y.,Koch, M.,Theodore, M.,Narsaria, U.,Shaevitz, J.,Gitai, Z.,Wu, Y.,Zhang, J.,Zeng, L.
Removal of Pseudomonas type IV pili by a small RNA virus.
Science, 384:eadl0635-eadl0635, 2024

PubMed Abstract: The retractile type IV pilus (T4P) is important for virulence of the opportunistic human pathogen . The single-stranded RNA (ssRNA) phage PP7 binds to T4P and is brought to the cell surface through pilus retraction. Using fluorescence microscopy, we discovered that PP7 detaches T4P, which impairs cell motility and restricts the pathogen's virulence. Using cryo-electron microscopy, mutagenesis, optical trapping, and Langevin dynamics simulation, we resolved the structure of PP7, T4P, and the PP7/T4P complex and showed that T4P detachment is driven by the affinity between the phage maturation protein and its bound pilin, plus the pilus retraction force and speed, and pilus bending. Pilus detachment may be widespread among other ssRNA phages and their retractile pilus systems and offers new prospects for antibacterial prophylaxis and therapeutics.

PubMed: 38574145
DOI: 10.1126/science.adl0635

実験手法

ELECTRON MICROSCOPY (3.6 Å)