8TU2

The Capsid of Rat Bocavirus

This is a non-PDB format compatible entry.

Summary for 8TU2

• Entry DOI: 10.2210/pdb8tu2/pdb
• EMDB information: 41614 41615 41616
• Descriptor: VP2 (1 entity in total)
• Functional Keywords: parvovirus, rbov, rat bocavirus, capsid, virus
• Biological source: Rat bocavirus
• Total number of polymer chains: 60
• Total formula weight: 3812444.04

Authors

Velez, M.,Mietzsch, M.,McKenna, R. (deposition date: 2023-08-15, release date: 2023-08-30, Last modification date: 2025-05-21)

Primary citation

Velez, M.,Mietzsch, M.,Hsi, J.,Bell, L.,Chipman, P.,Fu, X.,McKenna, R.
Structural Characterization of Canine Minute Virus, Rat and Porcine Bocavirus.
Viruses, 15:-, 2023

PubMed Abstract: is an expansive genus of the , with a wide range of vertebrate hosts. This study investigates Canine minute virus (CnMV), Rat bocavirus (RBoV), and Porcine bocavirus 1 (PBoV1). Both CnMV and PBoV1 have been found in gastrointestinal infections in their respective hosts, with CnMV responsible for spontaneous abortions in dogs, while PBoV has been associated with encephalomyelitis in piglets. The pathogenicity of the recently identified RBoV is currently unknown. To initiate the characterization of these viruses, their capsids structures were determined by cryo-electron microscopy at resolutions ranging from 2.3 to 2.7 Å. Compared to other parvoviruses, the CnMV, PBoV1, and RBoV capsids showed conserved features, such as the channel at the fivefold symmetry axis. However, major differences were observed at the two- and threefold axes. While CnMV displays prominent threefold protrusions, the same region is more recessed in PBoV1 and RBoV. Furthermore, the typical twofold axis depression of parvoviral capsids is absent in CnMV or very small in PBoV and RBoV. These capsid structures extend the structural portfolio for the genus and will allow future characterization of these pathogens on a molecular level. This is important, as no antivirals or vaccines exist for these viruses.

PubMed: 37766206
DOI: 10.3390/v15091799

Experimental method

ELECTRON MICROSCOPY (2.52 Å)