8TSE

Crystal structure of a CE15 glucuronoyl esterase from Ruminococcus flavefaciens

8TSE の概要

• エントリーDOI: 10.2210/pdb8tse/pdb
• 分子名称: Multidomain esterase, GLYCEROL (3 entities in total)
• 機能のキーワード: carbohydrate, esterase, ce15, glucuronoyl esterase, rumen, hydrolase
• 由来する生物種: Ruminococcus flavefaciens 17
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48245.98

構造登録者

Gruninger, R.J.,Jones, D.R. (登録日: 2023-08-11, 公開日: 2024-08-28, 最終更新日: 2024-09-18)

主引用文献

Gruninger, R.J.,Kevorkova, M.,Low, K.E.,Jones, D.R.,Worrall, L.,McAllister, T.A.,Abbott, D.W.
Structural, Biochemical, and Phylogenetic Analysis of Bacterial and Fungal Carbohydrate Esterase Family 15 Glucuronoyl Esterases in the Rumen.
Protein J., 43:910-922, 2024

PubMed Abstract: Glucuronoyl esterases (GEs) are carbohydrate active enzymes in carbohydrate esterase family 15 which are involved in the hydrolysis of lignin-carbohydrate complexes. They are encoded by a wide range of aerobic and anaerobic fungi and bacteria inhabiting diverse environments. The rumen microbiome is a complex microbial community with a wide array of enzymes that specialize in deconstructing plant cell wall carbohydrates. Enzymes from the rumen tend to show low similarity to homologues found in other environments, making the rumen microbiome a promising source for the discovery of novel enzymes. Using a combination of phylogenetic and structural analysis, we investigated the structure-function relationship of GEs from the rumen bacteria Fibrobacter succinogenes and Ruminococcus flavefaciens, and from the rumen fungus, Piromyces rhizinflata. All adopt a canonical α/β hydrolase fold and possess a structurally conserved Ser-His-Glu/Asp catalytic triad. Structural variations in the enzymes are localized to loops surrounding the active site. Analysis of the active site structures in these enzymes emphasized the importance of structural plasticity in GEs with non-canonical active site conformations. We hypothesize that interkingdom HGT events may have contributed to the diversity of GEs in the rumen, and this is demonstrated by the phylogenetic and structural similarity observed between rumen bacterial and fungal GEs. This study advances our understanding of the structure-function relationship in glucuronoyl esterases and illuminates the evolutionary dynamics that contribute to enzyme diversity in the rumen microbiome.

PubMed: 39153129
DOI: 10.1007/s10930-024-10221-0

実験手法

X-RAY DIFFRACTION (1.25 Å)