8TRD

mGluR3 class 1 in the presence of the antagonist LY 341495

8TRD の概要

• エントリーDOI: 10.2210/pdb8trd/pdb
• EMDBエントリー: 41578
• 分子名称: Metabotropic glutamate receptor 3, 2-[(1S,2S)-2-carboxycyclopropyl]-3-(9H-xanthen-9-yl)-D-alanine (2 entities in total)
• 機能のキーワード: gpcr, synaptic protein, membrane protein
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 207029.85

構造登録者

Strauss, A.,Levitz, J. (登録日: 2023-08-09, 公開日: 2024-07-31, 最終更新日: 2024-10-23)

主引用文献

Strauss, A.,Gonzalez-Hernandez, A.J.,Lee, J.,Abreu, N.,Selvakumar, P.,Salas-Estrada, L.,Kristt, M.,Arefin, A.,Huynh, K.,Marx, D.C.,Gilliland, K.,Melancon, B.J.,Filizola, M.,Meyerson, J.,Levitz, J.
Structural basis of positive allosteric modulation of metabotropic glutamate receptor activation and internalization.
Nat Commun, 15:6498-6498, 2024

PubMed Abstract: The metabotropic glutamate receptors (mGluRs) are neuromodulatory family C G protein coupled receptors which assemble as dimers and allosterically couple extracellular ligand binding domains (LBDs) to transmembrane domains (TMDs) to drive intracellular signaling. Pharmacologically, mGluRs can be targeted at the LBDs by glutamate and synthetic orthosteric compounds or at the TMDs by allosteric modulators. Despite the potential of allosteric compounds as therapeutics, an understanding of the functional and structural basis of their effects is limited. Here we use multiple approaches to dissect the functional and structural effects of orthosteric versus allosteric ligands. We find, using electrophysiological and live cell imaging assays, that both agonists and positive allosteric modulators (PAMs) can drive activation and internalization of group II and III mGluRs. The effects of PAMs are pleiotropic, boosting the maximal response to orthosteric agonists and serving independently as internalization-biased agonists across mGluR subtypes. Motivated by this and intersubunit FRET analyses, we determine cryo-electron microscopy structures of mGluR3 in the presence of either an agonist or antagonist alone or in combination with a PAM. These structures reveal PAM-driven re-shaping of intra- and inter-subunit conformations and provide evidence for a rolling TMD dimer interface activation pathway that controls G protein and beta-arrestin coupling.

PubMed: 39090128
DOI: 10.1038/s41467-024-50548-x

実験手法

ELECTRON MICROSCOPY (3.4 Å)