8TNV

Hemocyanin Functional Unit CCHB-g of Concholepas concholepas

8TNV の概要

• エントリーDOI: 10.2210/pdb8tnv/pdb
• 分子名称: Hemocyanin Functional Unit CCHB-g, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: hemocyanin, metalloprotein, copper protein, concholepas concholepas, oxygen transport
• 由来する生物種: Concholepas concholepas
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93616.50

構造登録者

Munoz, S.,Vallejos-Baccelliere, G.,Manubens, A.,Salazar, M.,Nascimento, A.F.Z.,Ambrosio, A.L.B.,Becker, M.I.,Guixe, V.,Castro-Fernandez, V. (登録日: 2023-08-02, 公開日: 2024-04-10, 最終更新日: 2024-11-20)

主引用文献

Munoz, S.M.,Vallejos-Baccelliere, G.,Manubens, A.,Salazar, M.L.,Nascimento, A.F.Z.,Tapia-Reyes, P.,Meneses, C.,Ambrosio, A.L.B.,Becker, M.I.,Guixe, V.,Castro-Fernandez, V.
Structural insights into a functional unit from an immunogenic mollusk hemocyanin.
Structure, 32:812-823.e4, 2024

PubMed Abstract: Mollusk hemocyanins, among the largest known proteins, are used as immunostimulants in biomedical and clinical applications. The hemocyanin of the Chilean gastropod Concholepas concholepas (CCH) exhibits unique properties, which makes it safe and effective for human immunotherapy, as observed in animal models of bladder cancer and melanoma, and dendritical cell vaccine trials. Despite its potential, the structure and amino acid sequence of CCH remain unknown. This study reports two sequence fragments of CCH, representing three complete functional units (FUs). We also determined the high-resolution (1.5 Å) X-ray crystal structure of an "FU-g type" from the CCHB subunit. This structure enables in-depth analysis of chemical interactions at the copper-binding center and unveils an unusual, truncated N-glycosylation pattern. These features are linked to eliciting more robust immunological responses in animals, offering insights into CCH's enhanced immunostimulatory properties and opening new avenues for its potential applications in biomedical research and therapies.

PubMed: 38513659
DOI: 10.1016/j.str.2024.02.018

実験手法

X-RAY DIFFRACTION (1.5 Å)