8TNS

Solution structure of poly(UG) RNA (GU)12 G-quadruplex

8TNS の概要

• エントリーDOI: 10.2210/pdb8tns/pdb
• NMR情報: BMRB: 52045
• 分子名称: RNA (5'-R(*GP*UP*GP*UP*GP*UP*GP*UP*GP*UP*GP*UP*GP*UP*GP*UP*GP*UP*GP*UP*GP*UP*GP*U)-3') (1 entity in total)
• 機能のキーワード: g-quadruplex, p(ug)-fold, rna
• 由来する生物種: Caenorhabditis elegans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7771.50

構造登録者

Escobar, C.A.,Petersen, R.,Butcher, S.E. (登録日: 2023-08-02, 公開日: 2023-11-15, 最終更新日: 2024-05-15)

主引用文献

Escobar, C.A.,Petersen, R.J.,Tonelli, M.,Fan, L.,Henzler-Wildman, K.A.,Butcher, S.E.
Solution Structure of Poly(UG) RNA.
J.Mol.Biol., 435:168340-168340, 2023

PubMed Abstract: Poly(UG) or "pUG" RNAs are UG or GU dinucleotide repeat sequences which are highly abundant in eukaryotes. Post-transcriptional addition of pUGs to RNA 3' ends marks mRNAs as vectors for gene silencing in C. elegans. We previously determined the crystal structure of pUG RNA bound to the ligand N-methyl mesoporphyrin IX (NMM), but the structure of free pUG RNA is unknown. Here we report the solution structure of the free pUG RNA (GU), as determined by nuclear magnetic resonance spectroscopy and small and wide-angle x-ray scattering (NMR-SAXS-WAXS). The low complexity sequence and 4-fold symmetry of the structure result in overlapped NMR signals that complicate chemical shift assignment. We therefore utilized single site-specific deoxyribose modifications which did not perturb the structure and introduced well-resolved methylene signals that are easily identified in NMR spectra. The solution structure ensemble has a root mean squared deviation (RMSD) of 0.62 Å and is a compact, left-handed quadruplex with a Z-form backbone, or "pUG fold." Overall, the structure agrees with the crystal structure of (GU) bound to NMM, indicating the pUG fold is unaltered by docking of the NMM ligand. The solution structure reveals conformational details that could not be resolved by x-ray crystallography, which explain how the pUG fold can form within longer RNAs.

PubMed: 37924862
DOI: 10.1016/j.jmb.2023.168340

実験手法

SOLUTION NMR
SOLUTION SCATTERING