8TMX

Crystal structure of engineered Castilleja foliolosa KAI2d15 variant

Summary for 8TMX

Entry DOI	10.2210/pdb8tmx/pdb
Descriptor	KAI2d15, GLYCEROL (3 entities in total)
Functional Keywords	strigolactone receptor, hydrolase
Biological source	Castilleja foliolosa
Total number of polymer chains	2
Total formula weight	60535.39
Authors	Burger, M.,Chory, J. (deposition date: 2023-07-31, release date: 2024-12-11, Last modification date: 2025-01-22)
Primary citation	Burger, M.,Peterson, D.,Chory, J. Strigolactones initiate the formation of haustorium-like structures in Castilleja . Iscience, 27:111491-111491, 2024 Cited by PubMed Abstract: Strigolactones serve as germination signals for several root-parasitic plant species within the Orobanchaceae family. Yet, their role in the life cycle of the facultatively parasitic genus has remained elusive. Here, we demonstrate that strigolactones initiate the formation of haustorium-like structures in . We present the genome of , which reveals an abundance of KAI2d paralogs that act as strigolactone receptors. 's KAI2d proteins include high-turnover enzymes, in particular KAI2d15, which features a substrate binding pocket with a methionine cluster at its entrance that is involved in the trade-off between substrate turnover and affinity. Our findings provide insights into strigolactone perception in and refine our perspective on their role in facultative parasitism. PubMed: 39759020 DOI: 10.1016/j.isci.2024.111491 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.9 Å)

Structure validation