8TLJ

CDCA7 (Mouse) Binds Non-B-form 32-mer DNA oligo Containing a 5mC

8TLJ の概要

• エントリーDOI: 10.2210/pdb8tlj/pdb
• 分子名称: Cell division cycle-associated protein 7, DNA (32-MER), GLYCEROL, ... (7 entities in total)
• 機能のキーワード: protein-dna complex, dna binding protein, zinc fingers, chromatin architecture, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 53599.90

構造登録者

Horton, J.R.,Ren, R.,Cheng, X. (登録日: 2023-07-26, 公開日: 2024-08-21, 最終更新日: 2024-08-28)

主引用文献

Hardikar, S.,Ren, R.,Ying, Z.,Horton, J.R.,Bramble, M.D.,Liu, B.,Lu, Y.,Liu, B.,Dan, J.,Zhang, X.,Cheng, X.,Chen, T.
The ICF syndrome protein CDCA7 harbors a unique DNA-binding domain that recognizes a CpG dyad in the context of a non-B DNA.
Biorxiv, 2023

PubMed Abstract: , encoding a protein with a C-terminal cysteine-rich domain (CRD), is mutated in immunodeficiency, centromeric instability and facial anomalies (ICF) syndrome, a disease related to hypomethylation of juxtacentromeric satellite DNA. How CDCA7 directs DNA methylation to juxtacentromeric regions is unknown. Here, we show that the CDCA7 CRD adopts a unique zinc-binding structure that recognizes a CpG dyad in a non-B DNA formed by two sequence motifs. CDCA7, but not ICF mutants, preferentially binds the non-B DNA with strand-specific CpG hemi-methylation. The unmethylated sequence motif is highly enriched at centromeres of human chromosomes, whereas the methylated motif is distributed throughout the genome. At S phase, CDCA7, but not ICF mutants, is concentrated in constitutive heterochromatin foci, and the formation of such foci can be inhibited by exogenous hemi-methylated non-B DNA bound by the CRD. Binding of the non-B DNA formed in juxtacentromeric regions during DNA replication provides a mechanism by which CDCA7 controls the specificity of DNA methylation.

PubMed: 38168392
DOI: 10.1101/2023.12.15.571946

実験手法

X-RAY DIFFRACTION (2.3 Å)