8TK9

ZIG-4-INS-6 complex, tetragonal form

Summary for 8TK9

• Entry DOI: 10.2210/pdb8tk9/pdb
• Descriptor: Zwei Ig domain protein zig-4, Probable insulin-like peptide beta-type 5, GLYCEROL, ... (5 entities in total)
• Functional Keywords: neuropeptide, signaling protein, protein binding, signaling protein-protein binding complex, signaling protein/protein binding
• Biological source: Caenorhabditis elegans; More
• Total number of polymer chains: 2
• Total formula weight: 30375.11

Authors

Cheng, S.,Baltrusaitis, E.,Aziz, Z.,Nawrocka, W.I.,Ozkan, E. (deposition date: 2023-07-25, release date: 2024-08-28, Last modification date: 2024-10-16)

Primary citation

Nawrocka, W.I.,Cheng, S.,Hao, B.,Rosen, M.C.,Cortes, E.,Baltrusaitis, E.E.,Aziz, Z.,Kovacs, I.A.,Ozkan, E.
Nematode Extracellular Protein Interactome Expands Connections between Signaling Pathways.
Biorxiv, 2024

PubMed Abstract: Multicellularity was accompanied by the emergence of new classes of cell surface and secreted proteins. The nematode is a favorable model to study cell surface interactomes, given its well-defined and stereotyped cell types and intercellular contacts. Here we report our extracellular interactome dataset, the largest yet for an invertebrate. Most of these interactions were unknown, despite recent datasets for flies and humans, as our collection contains a larger selection of protein families. We uncover new interactions for all four major axon guidance pathways, including ectodomain interactions between three of the pathways. We demonstrate that a protein family known to maintain axon locations are secreted receptors for insulins. We reveal novel interactions of cystine-knot proteins with putative signaling receptors, which may extend the study of neurotrophins and growth-factor-mediated functions to nematodes. Finally, our dataset provides insights into human disease mechanisms and how extracellular interactions may help establish connectomes.

PubMed: 39026773
DOI: 10.1101/2024.07.08.602367

Experimental method

X-RAY DIFFRACTION (1.3 Å)