8TK0

Structure of Gabija AB complex

8TK0 の概要

• エントリーDOI: 10.2210/pdb8tk0/pdb
• EMDBエントリー: 41314 41319 41321
• 分子名称: Endonuclease GajA (1 entity in total)
• 機能のキーワード: anti-phage defense, tetramer, dna recognition and cleavage, viral infection, bacterial immune system, immune system
• 由来する生物種: Bacillus cereus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 268317.88

構造登録者

Shen, Z.F.,Yang, X.Y.,Fu, T.M. (登録日: 2023-07-24, 公開日: 2024-04-24, 最終更新日: 2025-05-28)

主引用文献

Yang, X.Y.,Shen, Z.,Xie, J.,Greenwald, J.,Marathe, I.,Lin, Q.,Xie, W.J.,Wysocki, V.H.,Fu, T.M.
Molecular basis of Gabija anti-phage supramolecular assemblies.
Nat.Struct.Mol.Biol., 31:1243-1250, 2024

PubMed Abstract: As one of the most prevalent anti-phage defense systems in prokaryotes, Gabija consists of a Gabija protein A (GajA) and a Gabija protein B (GajB). The assembly and function of the Gabija system remain unclear. Here we present cryo-EM structures of Bacillus cereus GajA and GajAB complex, revealing tetrameric and octameric assemblies, respectively. In the center of the complex, GajA assembles into a tetramer, which recruits two sets of GajB dimer at opposite sides of the complex, resulting in a 4:4 GajAB supramolecular complex for anti-phage defense. Further biochemical analysis showed that GajA alone is sufficient to cut double-stranded DNA and plasmid DNA, which can be inhibited by ATP. Unexpectedly, the GajAB displays enhanced activity for plasmid DNA, suggesting a role of substrate selection by GajB. Together, our study defines a framework for understanding anti-phage immune defense by the GajAB complex.

PubMed: 38627580
DOI: 10.1038/s41594-024-01283-w

実験手法

ELECTRON MICROSCOPY (3.23 Å)