8TIZ

Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with 2 sticky base overhangs and no terminal phosphates and crosslinked with EDC.

Summary for 8TIZ

• Entry DOI: 10.2210/pdb8tiz/pdb
• Related: 7u6k
• Descriptor: DNA (5'-D(CP*CP*CP*GP*GP*AP*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*AP*CP*GP*G)-3')|, DNA (5'-D(GP*GP*CP*CP*GP*TP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*TP*CP*CP*GP)-3'), Replication initiation protein, ... (5 entities in total)
• Functional Keywords: replication initiator repe complex co-crystal, dna binding protein-dna complex, dna binding protein, dna binding protein/dna
• Biological source: Escherichia coli K-12; More
• Total number of polymer chains: 3
• Total formula weight: 49914.52

Authors

Orun, A.R.,Shields, E.T.,Shrestha, R.,Slaughter, C.K.,Snow, C.D. (deposition date: 2023-07-20, release date: 2023-08-02, Last modification date: 2024-08-14)

• Primary citation: Orun, A.R.,Slaughter, C.K.,Shields, E.T.,Vajapayajula, A.,Jones, S.,Shrestha, R.,Snow, C.D.; Tuning chemical DNA ligation within DNA crystals and protein-DNA co-crystals; Acs Nanosci Au, 2024; DOI: 10.1021/acsnanoscienceau.4c00013

Experimental method

X-RAY DIFFRACTION (3.11 Å)