8TEO

Shaker in low K+ (4mM K+)

8TEO の概要

• エントリーDOI: 10.2210/pdb8teo/pdb
• EMDBエントリー: 41193
• 分子名称: Potassium voltage-gated channel protein Shaker, (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: voltage-gated potassium channel, membrane protein
• 由来する生物種: Drosophila melanogaster (fruit fly)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 322504.44

構造登録者

Tan, X.,Swartz, K.J. (登録日: 2023-07-06, 公開日: 2023-12-20)

主引用文献

Stix, R.,Tan, X.F.,Bae, C.,Fernandez-Marino, A.I.,Swartz, K.J.,Faraldo-Gomez, J.D.
Eukaryotic Kv channel Shaker inactivates through selectivity filter dilation rather than collapse.
Sci Adv, 9:eadj5539-eadj5539, 2023

PubMed Abstract: Eukaryotic voltage-gated K channels have been extensively studied, but the structural bases for some of their most salient functional features remain to be established. C-type inactivation, for example, is an auto-inhibitory mechanism that confers temporal resolution to their signal-firing activity. In a recent breakthrough, studies of a mutant of Shaker that is prone to inactivate indicated that this process entails a dilation of the selectivity filter, the narrowest part of the ion conduction pathway. Here, we report an atomic-resolution cryo-electron microscopy structure that demonstrates that the wild-type channel can also adopt this dilated state. All-atom simulations corroborate this conformation is congruent with the electrophysiological characteristics of the C-type inactivated state, namely, residual K conductance and altered ion specificity, and help rationalize why inactivation is accelerated or impeded by certain mutations. In summary, this study establishes the molecular basis for an important self-regulatory mechanism in eukaryotic K channels, laying a solid foundation for further studies.

PubMed: 38064553
DOI: 10.1126/sciadv.adj5539

実験手法

ELECTRON MICROSCOPY (2.39 Å)