8TAF

Autographa californica multiple nucleopolyhedrovirus VP39

8TAF の概要

• エントリーDOI: 10.2210/pdb8taf/pdb
• EMDBエントリー: 41133
• 分子名称: Major viral capsid protein, ZINC ION (2 entities in total)
• 機能のキーワード: nucleocapsid protein vp39, viral protein
• 由来する生物種: Autographa californica multiple nucleopolyhedrovirus
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 286870.65

構造登録者

Benning, F.M.C.,Chao, L.H. (登録日: 2023-06-27, 公開日: 2024-01-10, 最終更新日: 2025-05-28)

主引用文献

Benning, F.M.C.,Jenni, S.,Garcia, C.Y.,Nguyen, T.H.,Zhang, X.,Chao, L.H.
Helical reconstruction of VP39 reveals principles for baculovirus nucleocapsid assembly.
Nat Commun, 15:250-250, 2024

PubMed Abstract: Baculoviruses are insect-infecting pathogens with wide applications as biological pesticides, in vitro protein production vehicles and gene therapy tools. Its cylindrical nucleocapsid, which encapsulates and protects the circular double-stranded viral DNA encoding proteins for viral replication and entry, is formed by the highly conserved major capsid protein VP39. The mechanism for VP39 assembly remains unknown. We use electron cryomicroscopy to determine a 3.2 Å helical reconstruction of an infectious nucleocapsid of Autographa californica multiple nucleopolyhedrovirus, revealing how dimers of VP39 assemble into a 14-stranded helical tube. We show that VP39 comprises a distinct protein fold conserved across baculoviruses, which includes a Zinc finger domain and a stabilizing intra-dimer sling. Analysis of sample polymorphism shows that VP39 assembles in several closely-related helical geometries. This VP39 reconstruction reveals general principles for baculoviral nucleocapsid assembly.

PubMed: 38177118
DOI: 10.1038/s41467-023-44596-y

実験手法

ELECTRON MICROSCOPY (3.2 Å)