8T53

S. enterica WbaP in a styrene maleic acid liponanoparticle

8T53 の概要

• エントリーDOI: 10.2210/pdb8t53/pdb
• EMDBエントリー: 41042
• 分子名称: Undecaprenyl-phosphate galactose phosphotransferase (1 entity in total)
• 機能のキーワード: phosphoglycosyl transferase, liponanopaticle, smalp, o-antigen, salmonella enterica, transferase
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhimurium
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 122765.40

構造登録者

Dodge, G.J.,Imperiali, B. (登録日: 2023-06-12, 公開日: 2024-02-14, 最終更新日: 2024-02-28)

主引用文献

Dodge, G.J.,Anderson, A.J.,He, Y.,Liu, W.,Viner, R.,Imperiali, B.
Mapping the architecture of the initiating phosphoglycosyl transferase from S. enterica O-antigen biosynthesis in a liponanoparticle.
Elife, 12:-, 2024

PubMed Abstract: Bacterial cell surface glycoconjugates are critical for cell survival and for interactions between bacteria and their hosts. Consequently, the pathways responsible for their biosynthesis have untapped potential as therapeutic targets. The localization of many glycoconjugate biosynthesis enzymes to the membrane represents a significant challenge for expressing, purifying, and characterizing these enzymes. Here, we leverage cutting-edge detergent-free methods to stabilize, purify, and structurally characterize WbaP, a phosphoglycosyl transferase (PGT) from the (LT2) O-antigen biosynthesis. From a functional perspective, these studies establish WbaP as a homodimer, reveal the structural elements responsible for dimerization, shed light on the regulatory role of a domain of unknown function embedded within WbaP, and identify conserved structural motifs between PGTs and functionally unrelated UDP-sugar dehydratases. From a technological perspective, the strategy developed here is generalizable and provides a toolkit for studying other classes of small membrane proteins embedded in liponanoparticles beyond PGTs.

PubMed: 38358918
DOI: 10.7554/eLife.91125

実験手法

ELECTRON MICROSCOPY (4.1 Å)