8T4D の概要
| エントリーDOI | 10.2210/pdb8t4d/pdb |
| EMDBエントリー | 41027 |
| 分子名称 | MD65 N332-GT5 SOSIP gp120, RM20A3 heavy chain Fv, RM20A3 light chain Fv, ... (9 entities in total) |
| 機能のキーワード | germline targeting, nhp, sosip, env, hiv, bg18, immunogen design, antibody, viral protein, viral protein-immune system complex, viral protein/immune system |
| 由来する生物種 | Human immunodeficiency virus 1 詳細 |
| タンパク質・核酸の鎖数 | 18 |
| 化学式量合計 | 389388.35 |
| 構造登録者 | |
| 主引用文献 | Steichen, J.M.,Phung, I.,Salcedo, E.,Ozorowski, G.,Willis, J.R.,Baboo, S.,Liguori, A.,Cottrell, C.A.,Torres, J.L.,Madden, P.J.,Ma, K.M.,Sutton, H.J.,Lee, J.H.,Kalyuzhniy, O.,Allen, J.D.,Rodriguez, O.L.,Adachi, Y.,Mullen, T.M.,Georgeson, E.,Kubitz, M.,Burns, A.,Barman, S.,Mopuri, R.,Metz, A.,Altheide, T.K.,Diedrich, J.K.,Saha, S.,Shields, K.,Schultze, S.E.,Smith, M.L.,Schiffner, T.,Burton, D.R.,Watson, C.T.,Bosinger, S.E.,Crispin, M.,Yates 3rd, J.R.,Paulson, J.C.,Ward, A.B.,Sok, D.,Crotty, S.,Schief, W.R. Vaccine priming of rare HIV broadly neutralizing antibody precursors in nonhuman primates. Science, 384:eadj8321-eadj8321, 2024 Cited by PubMed Abstract: Germline-targeting immunogens hold promise for initiating the induction of broadly neutralizing antibodies (bnAbs) to HIV and other pathogens. However, antibody-antigen recognition is typically dominated by heavy chain complementarity determining region 3 (HCDR3) interactions, and vaccine priming of HCDR3-dominant bnAbs by germline-targeting immunogens has not been demonstrated in humans or outbred animals. In this work, immunization with N332-GT5, an HIV envelope trimer designed to target precursors of the HCDR3-dominant bnAb BG18, primed bnAb-precursor B cells in eight of eight rhesus macaques to substantial frequencies and with diverse lineages in germinal center and memory B cells. We confirmed bnAb-mimicking, HCDR3-dominant, trimer-binding interactions with cryo-electron microscopy. Our results demonstrate proof of principle for HCDR3-dominant bnAb-precursor priming in outbred animals and suggest that N332-GT5 holds promise for the induction of similar responses in humans. PubMed: 38753769DOI: 10.1126/science.adj8321 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.1 Å) |
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