8T2E

BG505 Boost2 SOSIP.664 in complex with NHP polyclonal antibody FP3

これはPDB形式変換不可エントリーです。

8T2E の概要

• エントリーDOI: 10.2210/pdb8t2e/pdb
• 関連するPDBエントリー: 8SWV
• EMDBエントリー: 40981
• 分子名称: Surface protein gp120, Transmembrane protein gp41, FP3 Heavy Chain, ... (7 entities in total)
• 機能のキーワード: hiv-1, env, nhp, antibody, fusion peptide, polyclonal, viral protein
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 259551.62

構造登録者

Pratap, P.P.,Antansijevic, A.,Ozorowski, G.,Ward, A.B. (登録日: 2023-06-05, 公開日: 2024-06-12, 最終更新日: 2025-05-28)

主引用文献

Cottrell, C.A.,Pratap, P.P.,Cirelli, K.M.,Carnathan, D.G.,Enemuo, C.A.,Antanasijevic, A.,Ozorowski, G.,Sewall, L.M.,Gao, H.,Allen, J.D.,Nogal, B.,Silva, M.,Bhiman, J.,Pauthner, M.,Irvine, D.J.,Montefiori, D.,Crispin, M.,Burton, D.R.,Silvestri, G.,Crotty, S.,Ward, A.B.
Priming antibody responses to the fusion peptide in rhesus macaques.
Npj Vaccines, 9:126-126, 2024

PubMed Abstract: Immunodominance of antibodies targeting non-neutralizing epitopes and the high level of somatic hypermutation within germinal centers (GCs) required for most HIV broadly neutralizing antibodies (bnAbs) are major impediments to the development of an effective HIV vaccine. Rational protein vaccine design and non-conventional immunization strategies are potential avenues to overcome these hurdles. Here, we report using implantable osmotic pumps to continuously deliver a series of epitope-targeted immunogens to rhesus macaques over the course of six months to prime and elicit antibody responses against the conserved fusion peptide (FP). GC responses and antibody specificities were tracked longitudinally using lymph node fine-needle aspirates and electron microscopy polyclonal epitope mapping (EMPEM), respectively, to show antibody responses to the FP/N611 glycan hole region were primed, although exhibited limited neutralization breadth. Application of cryoEMPEM delineated key residues for on-target and off-target responses that can drive the next round of structure-based vaccine design.

PubMed: 38997302
DOI: 10.1038/s41541-024-00918-9

実験手法

ELECTRON MICROSCOPY (3.5 Å)