8T07

Structure of mouse Myomaker mutant-Y118A bound to Fab18G7

8T07 の概要

• エントリーDOI: 10.2210/pdb8t07/pdb
• EMDBエントリー: 40937
• 分子名称: Protein myomaker, 18G7 Fab heavy chain, 18G7 Fab light chain, ... (4 entities in total)
• 機能のキーワード: membrane protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 99837.95

構造登録者

Long, T.,Li, X. (登録日: 2023-05-31, 公開日: 2023-09-27, 最終更新日: 2024-10-16)

主引用文献

Long, T.,Zhang, Y.,Donnelly, L.,Li, H.,Pien, Y.C.,Liu, N.,Olson, E.N.,Li, X.
Cryo-EM structures of Myomaker reveal a molecular basis for myoblast fusion.
Nat.Struct.Mol.Biol., 30:1746-1754, 2023

PubMed Abstract: The fusion of mononucleated myoblasts produces multinucleated muscle fibers leading to the formation of skeletal muscle. Myomaker, a skeletal muscle-specific membrane protein, is essential for myoblast fusion. Here we report the cryo-EM structures of mouse Myomaker (mMymk) and Ciona robusta Myomaker (cMymk). Myomaker contains seven transmembrane helices (TMs) that adopt a G-protein-coupled receptor-like fold. TMs 2-4 form a dimeric interface, while TMs 3 and 5-7 create a lipid-binding site that holds the polar head of a phospholipid and allows the alkyl tails to insert into Myomaker. The similarity of cMymk and mMymk suggests a conserved Myomaker-mediated cell fusion mechanism across evolutionarily distant species. Functional analyses demonstrate the essentiality of the dimeric interface and the lipid-binding site for fusogenic activity, and heterologous cell-cell fusion assays show the importance of transcellular interactions of Myomaker protomers for myoblast fusion. Together, our findings provide structural and functional insights into the process of myoblast fusion.

PubMed: 37770716
DOI: 10.1038/s41594-023-01110-8

実験手法

ELECTRON MICROSCOPY (3.38 Å)