8SYI

Cyanobacterial RNAP-EC

8SYI の概要

• エントリーDOI: 10.2210/pdb8syi/pdb
• EMDBエントリー: 40874
• 分子名称: DNA-directed RNA polymerase subunit alpha, MAGNESIUM ION, ZINC ION, ... (11 entities in total)
• 機能のキーワード: transcription regulation rnap nusg cryo-em, transcription
• 由来する生物種: Synechococcus elongatus; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 469143.78

構造登録者

Qayyum, M.Z.,Imashimizu, M.,Leanca, M.,Vishwakarma, R.K.,Bradley Riaz, A.,Yuzenkova, Y.,Murakami, K.S. (登録日: 2023-05-25, 公開日: 2023-07-05, 最終更新日: 2024-02-21)

主引用文献

Qayyum, M.Z.,Imashimizu, M.,Leanca, M.,Vishwakarma, R.K.,Riaz-Bradley, A.,Yuzenkova, Y.,Murakami, K.S.
Structure and function of the Si3 insertion integrated into the trigger loop/helix of cyanobacterial RNA polymerase.
Proc.Natl.Acad.Sci.USA, 121:e2311480121-e2311480121, 2024

PubMed Abstract: Cyanobacteria and evolutionarily related chloroplasts of algae and plants possess unique RNA polymerases (RNAPs) with characteristics that distinguish them from canonical bacterial RNAPs. The largest subunit of cyanobacterial RNAP (cyRNAP) is divided into two polypeptides, β'1 and β'2, and contains the largest known lineage-specific insertion domain, Si3, located in the middle of the trigger loop and spanning approximately half of the β'2 subunit. In this study, we present the X-ray crystal structure of Si3 and the cryo-EM structures of the cyRNAP transcription elongation complex plus the NusG factor with and without incoming nucleoside triphosphate (iNTP) bound at the active site. Si3 has a well-ordered and elongated shape that exceeds the length of the main body of cyRNAP, fits into cavities of cyRNAP in the absence of iNTP bound at the active site and shields the binding site of secondary channel-binding proteins such as Gre and DksA. A small transition from the trigger loop to the trigger helix upon iNTP binding results in a large swing motion of Si3; however, this transition does not affect the catalytic activity of cyRNAP due to its minimal contact with cyRNAP, NusG, or DNA. This study provides a structural framework for understanding the evolutionary significance of these features unique to cyRNAP and chloroplast RNAP and may provide insights into the molecular mechanism of transcription in specific environment of photosynthetic organisms and organelle.

PubMed: 38354263
DOI: 10.1073/pnas.2311480121

実験手法

ELECTRON MICROSCOPY (2.94 Å)