8SY9

X-ray crystal structure of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from Thermus thermophilus strain HB27, D98N variant in the presence of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid, UDP-N-acetylglucosamine and UDP at pH 7

Summary for 8SY9

• Entry DOI: 10.2210/pdb8sy9/pdb
• Related: 8SXV 8SXW 8SXY 8SY0
• Descriptor: UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase, (2~{S},3~{S},4~{R},5~{R},6~{R})-4,5-diacetamido-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxane-2-carboxylic acid, URIDINE-5'-DIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: lipopolysaccharide, o-antigen, 2-epimerase, isomerase
• Biological source: Thermus thermophilus HB27
• Total number of polymer chains: 2
• Total formula weight: 86324.47

Authors

Thoden, J.B.,Holden, H.M. (deposition date: 2023-05-25, release date: 2023-09-13, Last modification date: 2023-10-18)

• Primary citation: Thoden, J.B.,McKnight, J.O.,Kroft, C.W.,Jast, J.D.T.,Holden, H.M.; Structural analysis of a bacterial UDP-sugar 2-epimerase reveals the active site architecture before and after catalysis.; J.Biol.Chem., 299:105200-105200, 2023; PubMed: 37660908; DOI: 10.1016/j.jbc.2023.105200

Experimental method

X-RAY DIFFRACTION (2.2 Å)