8SVB
Antimicrobial lasso peptide achromonodin-1
Summary for 8SVB
| Entry DOI | 10.2210/pdb8svb/pdb |
| NMR Information | BMRB: 31086 |
| Descriptor | Achromonodin-1 (1 entity in total) |
| Functional Keywords | antimicrobial, lasso peptide, antibiotic |
| Biological source | Achromobacter xylosoxidans |
| Total number of polymer chains | 1 |
| Total formula weight | 3220.59 |
| Authors | Carson, D.V.,Cheung-Lee, W.L.,So, L.,Link, A.J. (deposition date: 2023-05-16, release date: 2023-10-11, Last modification date: 2024-10-30) |
| Primary citation | Carson, D.V.,Zhang, Y.,So, L.,Cheung-Lee, W.L.,Cartagena, A.J.,Darst, S.A.,Link, A.J. Discovery, Characterization, and Bioactivity of the Achromonodins: Lasso Peptides Encoded by Achromobacter . J.Nat.Prod., 86:2448-2456, 2023 Cited by PubMed Abstract: Through genome mining efforts, two lasso peptide biosynthetic gene clusters (BGCs) within two different species of , a genus that contains pathogenic organisms that can infect patients with cystic fibrosis, were discovered. Using gene-refactored BGCs in , these lasso peptides, which were named achromonodin-1 and achromonodin-2, were heterologously expressed. Achromonodin-1 is naturally encoded by certain isolates from the sputum of patients with cystic fibrosis. The NMR structure of achromonodin-1 was determined, demonstrating that it is a threaded lasso peptide with a large loop and short tail structure, reminiscent of previously characterized lasso peptides that inhibit RNA polymerase (RNAP). Achromonodin-1 inhibits RNAP and has potent, focused activity toward , another isolate from the sputum of a cystic fibrosis patient. These efforts expand the repertoire of antimicrobial lasso peptides and provide insights into how isolates from certain ecological niches interact with each other. PubMed: 37870195DOI: 10.1021/acs.jnatprod.3c00536 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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