8SNH

cytochrome bc1-cbb3 supercomplex from Pseudomonas aeruginosa

8SNH の概要

• エントリーDOI: 10.2210/pdb8snh/pdb
• EMDBエントリー: 40625
• 分子名称: cytochrome-c oxidase, CALCIUM ION, PROTOPORPHYRIN IX CONTAINING FE, ... (15 entities in total)
• 機能のキーワード: electron transport chain, supercomplex, pseudomonas aeruginosa, membrane protein
• 由来する生物種: Pseudomonas aeruginosa; 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 371910.80

構造登録者

Di Trani, J.M.,Rubinstein, J.L. (登録日: 2023-04-27, 公開日: 2023-10-11, 最終更新日: 2025-05-14)

主引用文献

Di Trani, J.M.,Gheorghita, A.A.,Turner, M.,Brzezinski, P.,Adelroth, P.,Vahidi, S.,Howell, P.L.,Rubinstein, J.L.
Structure of the bc 1 - cbb 3 respiratory supercomplex from Pseudomonas aeruginosa.
Proc.Natl.Acad.Sci.USA, 120:e2307093120-e2307093120, 2023

PubMed Abstract: Energy conversion by electron transport chains occurs through the sequential transfer of electrons between protein complexes and intermediate electron carriers, creating the proton motive force that enables ATP synthesis and membrane transport. These protein complexes can also form higher order assemblies known as respiratory supercomplexes (SCs). The electron transport chain of the opportunistic pathogen is closely linked with its ability to invade host tissue, tolerate harsh conditions, and resist antibiotics but is poorly characterized. Here, we determine the structure of a SC that forms between the quinol:cytochrome oxidoreductase (cytochrome ) and one of the organism's terminal oxidases, cytochrome , which is found only in some bacteria. Remarkably, the SC structure also includes two intermediate electron carriers: a diheme cytochrome and a single heme cytochrome . Together, these proteins allow electron transfer from ubiquinol in cytochrome to oxygen in cytochrome . We also present evidence that different isoforms of cytochrome can participate in formation of this SC without changing the overall SC architecture. Incorporating these different subunit isoforms into the SC would allow the bacterium to adapt to different environmental conditions. Bioinformatic analysis focusing on structural motifs in the SC suggests that cytochrome - SCs also exist in other bacterial pathogens.

PubMed: 37751552
DOI: 10.1073/pnas.2307093120

実験手法

ELECTRON MICROSCOPY (2.7 Å)