8SMD

Structure of Clostridium botulinum prophage Tad1 in complex with 1''-3' gcADPR

8SMD の概要

• エントリーDOI: 10.2210/pdb8smd/pdb
• 分子名称: ABC transporter ATPase, (2R,3R,3aS,5S,6R,7S,8R,11R,13S,15aR)-2-(6-amino-9H-purin-9-yl)-3,6,7,11,13-pentahydroxyoctahydro-2H,5H,11H,13H-5,8-epoxy-11lambda~5~,13lambda~5~-furo[2,3-g][1,3,5,9,2,4]tetraoxadiphosphacyclotetradecine-11,13-dione (3 entities in total)
• 機能のキーワード: gcadpr, thoeris, anti-phage, unknown function
• 由来する生物種: Clostridium botulinum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29744.76

構造登録者

Lu, A.,Yirmiya, E.,Leavitt, A.,Avraham, C.,Osterman, I.,Garb, J.,Antine, S.P.,Mooney, S.E.,Hobbs, S.J.,Amitai, G.,Sorek, R.,Kranzusch, P.J. (登録日: 2023-04-26, 公開日: 2023-11-22, 最終更新日: 2024-01-24)

主引用文献

Yirmiya, E.,Leavitt, A.,Lu, A.,Ragucci, A.E.,Avraham, C.,Osterman, I.,Garb, J.,Antine, S.P.,Mooney, S.E.,Hobbs, S.J.,Kranzusch, P.J.,Amitai, G.,Sorek, R.
Phages overcome bacterial immunity via diverse anti-defence proteins.
Nature, 625:352-359, 2024

PubMed Abstract: It was recently shown that bacteria use, apart from CRISPR-Cas and restriction systems, a considerable diversity of phage resistance systems, but it is largely unknown how phages cope with this multilayered bacterial immunity. Here we analysed groups of closely related Bacillus phages that showed differential sensitivity to bacterial defence systems, and discovered four distinct families of anti-defence proteins that inhibit the Gabija, Thoeris and Hachiman systems. We show that these proteins Gad1, Gad2, Tad2 and Had1 efficiently cancel the defensive activity when co-expressed with the respective defence system or introduced into phage genomes. Homologues of these anti-defence proteins are found in hundreds of phages that infect taxonomically diverse bacterial species. We show that the anti-Gabija protein Gad1 blocks the ability of the Gabija defence complex to cleave phage-derived DNA. Our data further reveal that the anti-Thoeris protein Tad2 is a 'sponge' that sequesters the immune signalling molecules produced by Thoeris TIR-domain proteins in response to phage infection. Our results demonstrate that phages encode an arsenal of anti-defence proteins that can disable a variety of bacterial defence mechanisms.

PubMed: 37992756
DOI: 10.1038/s41586-023-06869-w

実験手法

X-RAY DIFFRACTION (2.1 Å)