8SMA

SfbO with di-Manganese cofactor

8SMA の概要

• エントリーDOI: 10.2210/pdb8sma/pdb
• 分子名称: Amidohydrolase family protein, SULFATE ION, MANGANESE (II) ION, ... (4 entities in total)
• 機能のキーワード: monooxygenase, amidohydrolase-like, pf04909, oxidoreductase
• 由来する生物種: Litorilinea aerophila
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 170815.98

構造登録者

Liu, C.,Rittle, J. (登録日: 2023-04-25, 公開日: 2024-03-13)

主引用文献

Liu, C.,Powell, M.M.,Rao, G.,Britt, R.D.,Rittle, J.
Bioinformatic Discovery of a Cambialistic Monooxygenase.
J.Am.Chem.Soc., 146:1783-1788, 2024

PubMed Abstract: Dinuclear monooxygenases mediate challenging C-H bond oxidation reactions throughout nature. Many of these enzymes are presumed to exclusively utilize diiron cofactors. Herein we report the bioinformatic discovery of an orphan dinuclear monooxygenase that preferentially utilizes a heterobimetallic manganese-iron (Mn/Fe) cofactor to mediate an O-dependent C-H bond hydroxylation reaction. Unlike the structurally similar Mn/Fe-dependent monooxygenase AibH2, the diiron form of this enzyme (SfbO) exhibits a nascent enzymatic activity. This behavior raises the possibility that many other dinuclear monooxygenases may be endowed with the capacity to harness cofactors with a variable metal content.

PubMed: 38198693
DOI: 10.1021/jacs.3c12131

実験手法

X-RAY DIFFRACTION (2.18 Å)