8SL0

Structure of a bacterial gasdermin slinky-like oligomer

これはPDB形式変換不可エントリーです。

8SL0 の概要

• エントリーDOI: 10.2210/pdb8sl0/pdb
• EMDBエントリー: 40570
• 分子名称: Gasdermin bGSDM (1 entity in total)
• 機能のキーワード: viral mimicry, gasdermin, caspase, autoinhibition, pyroptosis, bats, immunity, cell death, immune system
• 由来する生物種: Vitiosangium sp. GDMCC 1.1324
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25566.25

構造登録者

Johnson, A.G.,Mayer, M.L.,Kranzusch, P.J. (登録日: 2023-04-20, 公開日: 2023-05-17, 最終更新日: 2025-05-28)

主引用文献

Johnson, A.G.,Mayer, M.L.,Schaefer, S.L.,McNamara-Bordewick, N.K.,Hummer, G.,Kranzusch, P.J.
Structure and assembly of a bacterial gasdermin pore.
Nature, 628:657-663, 2024

PubMed Abstract: In response to pathogen infection, gasdermin (GSDM) proteins form membrane pores that induce a host cell death process called pyroptosis. Studies of human and mouse GSDM pores have revealed the functions and architectures of assemblies comprising 24 to 33 protomers, but the mechanism and evolutionary origin of membrane targeting and GSDM pore formation remain unknown. Here we determine a structure of a bacterial GSDM (bGSDM) pore and define a conserved mechanism of pore assembly. Engineering a panel of bGSDMs for site-specific proteolytic activation, we demonstrate that diverse bGSDMs form distinct pore sizes that range from smaller mammalian-like assemblies to exceptionally large pores containing more than 50 protomers. We determine a cryo-electron microscopy structure of a Vitiosangium bGSDM in an active 'slinky'-like oligomeric conformation and analyse bGSDM pores in a native lipid environment to create an atomic-level model of a full 52-mer bGSDM pore. Combining our structural analysis with molecular dynamics simulations and cellular assays, our results support a stepwise model of GSDM pore assembly and suggest that a covalently bound palmitoyl can leave a hydrophobic sheath and insert into the membrane before formation of the membrane-spanning β-strand regions. These results reveal the diversity of GSDM pores found in nature and explain the function of an ancient post-translational modification in enabling programmed host cell death.

PubMed: 38509367
DOI: 10.1038/s41586-024-07216-3

実験手法

ELECTRON MICROSCOPY (3.3 Å)