8SKW

MicroED structure of d(CGCGCG)2 Z-DNA

Summary for 8SKW

• Entry DOI: 10.2210/pdb8skw/pdb
• Descriptor: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3'), SPERMINE (3 entities in total)
• Functional Keywords: z-dna, dna
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 3822.75

Authors

Haymaker, A.,Bardin, A.A.,Martynowycz, M.W.,Nannenga, B.L. (deposition date: 2023-04-20, release date: 2023-08-16, Last modification date: 2023-12-20)

Primary citation

Haymaker, A.,Bardin, A.A.,Gonen, T.,Martynowycz, M.W.,Nannenga, B.L.
Structure determination of a DNA crystal by MicroED.
Structure, 31:1499-1503.e2, 2023

PubMed Abstract: Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG) DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG) DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.

PubMed: 37541248
DOI: 10.1016/j.str.2023.07.005

Experimental method

ELECTRON CRYSTALLOGRAPHY (1.1 Å)