8SKW

MicroED structure of d(CGCGCG)2 Z-DNA

8SKW の概要

• エントリーDOI: 10.2210/pdb8skw/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3'), SPERMINE (3 entities in total)
• 機能のキーワード: z-dna, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 3822.75

構造登録者

Haymaker, A.,Bardin, A.A.,Martynowycz, M.W.,Nannenga, B.L. (登録日: 2023-04-20, 公開日: 2023-08-16, 最終更新日: 2023-12-20)

主引用文献

Haymaker, A.,Bardin, A.A.,Gonen, T.,Martynowycz, M.W.,Nannenga, B.L.
Structure determination of a DNA crystal by MicroED.
Structure, 31:1499-1503.e2, 2023

PubMed Abstract: Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG) DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG) DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.

PubMed: 37541248
DOI: 10.1016/j.str.2023.07.005

実験手法

ELECTRON CRYSTALLOGRAPHY (1.1 Å)