8SD1

Carbonic anhydrase II radiation damage RT 1-30

Summary for 8SD1

• Entry DOI: 10.2210/pdb8sd1/pdb
• Descriptor: Carbonic anhydrase 2, ZINC ION (3 entities in total)
• Functional Keywords: xfel, metal binding protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 29354.47

Authors

Combs, J.C.,Mckenna, R. (deposition date: 2023-04-06, release date: 2024-03-13)

Primary citation

Hull, J.A.,Lee, C.,Kim, J.K.,Lim, S.W.,Park, J.,Park, S.,Lee, S.J.,Park, G.,Eom, I.,Kim, M.,Hyun, H.,Combs, J.E.,Andring, J.T.,Lomelino, C.,Kim, C.U.,McKenna, R.
XFEL structure of carbonic anhydrase II: a comparative study of XFEL, NMR, X-ray and neutron structures.
Acta Crystallogr D Struct Biol, 80:194-202, 2024

PubMed Abstract: The combination of X-ray free-electron lasers (XFELs) with serial femtosecond crystallography represents cutting-edge technology in structural biology, allowing the study of enzyme reactions and dynamics in real time through the generation of `molecular movies'. This technology combines short and precise high-energy X-ray exposure to a stream of protein microcrystals. Here, the XFEL structure of carbonic anhydrase II, a ubiquitous enzyme responsible for the interconversion of CO and bicarbonate, is reported, and is compared with previously reported NMR and synchrotron X-ray and neutron single-crystal structures.

PubMed: 38411550
DOI: 10.1107/S2059798324000482

Experimental method

X-RAY DIFFRACTION (1.298 Å)